Method Development and Validation of Fluconazole and Ivermectin in pure and Combined Dosage form using UV by Q- absorption ratio and Vierotd’s method

Abstract

Multicomponent analysis involves simultaneous estimation of drug substances in combined dosage form. A comparative study between Q-Absorption ratio technique and Simultaneous Equation Method has been established for effective implication of the developed method. Numerous trials were performed as a part of solvent selection, both the drugs under study Fluconazole (FLN) and Ivermectin (IVR) have shown good solubility in methanol (Spectroscopic Grade). The absorption maxima (λmax) were found to be 261nm for FLU and 245nm for IVR respectively. Both the drugs were showing same extinction coefficient (Isosbestic point) at 261nm. A comparative study is proposed to be established for simultaneous equation and Q-absorption ratio method. Between the concentration and absorbance, calibration curves revealed a linear relationship. The regression line equation was established and identified r2 for Fluconazole and Ivermectin is 0.999 and 0.999. Fluconazole and Ivermectin were assessed by the validated method when each of the drug individually subjected to various stress conditions like concentrated acidic, basic, peroxide, excessive light and thermal. For estimation, the suggested approach was used for drug content in locally available marketed formulations which has proven successful for routine analysis of the same with the application of vierotd’s as well as Q-absorption method.