Handheld Device for Rapid Detection of miRNA based on a Ratiometric Transmittance Scheme

2020 IEEE International Instrumentation and Measurement Technology Conference (I2MTC) Pub Date : 2020-05-01 DOI:10.1109/I2MTC43012.2020.9129284

Jorge Losada Matias, Adrián Sánchez Visedo, M. V. Llopis, F. Ferrero, J. M. Costa-Fernández, M. T. Fernández-Argüelles, Juan Carlos Campo Rodríguez, A. Cabezuelo

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Abstract

MicroRNAs (miRNAs) are a novel class of biomarkers that can be present in different biological samples, which can be related to many diseases. Controlled aggregation of gold nanoparticles (GNPs) in the presence of miRNAs can be used to perform a rapid detection of miRNAs based on color change. Sometimes the color change can be observed with the naked eye; however, this is not always possible. This work proposes the design of a novel instrumental prototype that allows performance of simultaneous transmittance measurements at different wavelengths. Once the signals are obtained, the system calculates the 525nm/650nm transmittance ratio and the miRNA concentration, displaying and recording the results in a smartphone. This prototype has been validated to detect the miRNA "miR146a," which is found in higher concentrations in milk and tissues in cases of bovine mastitis.

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基于比率透过率方案的手持miRNA快速检测装置

MicroRNAs (miRNAs)是一类新的生物标志物，可存在于不同的生物样品中，与许多疾病有关。在存在mirna的情况下，金纳米颗粒(GNPs)的受控聚集可用于基于颜色变化对mirna进行快速检测。有时可以用肉眼观察到颜色的变化;然而，这并不总是可能的。这项工作提出了一种新的仪器原型的设计，允许在不同波长的同时透射率测量的性能。一旦获得信号，系统将计算525nm/650nm的透射比和miRNA浓度，并将结果显示并记录在智能手机上。该原型已被验证可以检测miRNA“miR146a”，该miRNA在奶牛乳腺炎病例的牛奶和组织中含量较高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2020 IEEE International Instrumentation and Measurement Technology Conference (I2MTC)

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