IDENTIFICATION OF TANNINS IN THE LEAVES OF THE SQUAT CLOUDBERRY, GROWING IN THE VOLOGDA REGION OF RUSSIA

Kubasova E.D., Krylov I.A., Korelskaya G.V., Hlopina V.S., Kubasov R.V.
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Abstract

Abstract. Relevance: Squat cloudberry (Rubus chamaemorus) has a sufficiently wide range of therapeutic effects for various diseases. At the same time, it does not refer to pharmacopoeia medicinal plants, but is used as folk medicine. Tannins (plant tannins) detected in Rubus chamaemorus hold a special place among the other biologically active substances of this plant. They have anti-inflammatory, antimicrobial, antioxidant and anti-cancer activity. Also, their properties are applicable in the prevention of cardiovascular and metabolic diseases. The aim of the study is to establish the presence of tannins and their quantitative determination in the leafs of Cloudberry squat, growing in the Vologda region for the possible determination of the raw materials of this plant as medicinal. Materials and methods: the presence and quantitative content of tannins in the raw materials of the squat cloudberry (leaves) growing on the territory of the Vologda region was determined using various methods of chemical analysis. Results of the study: qualitative samples confirmed the presence of tannins in the leaves of the studied plant (gelatin sample), as well as the sufficient presence of their hydrolyzable forms (iron-ammonium alum sample); quantitative (by permanganatometry) determined the content of tannins relative to the total dry weight of raw materials, it was 15.03 ± 1.14% (95% confidence interval = 14.12-15.95). The results obtained are consistent with the data of other researchers, the average relative proportion of tannins in relation to the total mass of the dry plant correlates to the average scientific results. The conclusion is made about the possibility of attributing Rubus chamaemorus, which grows in the Vologda region, to medicinal plants potentially recommended for use in the pharmaceutical industry.
生长在俄罗斯沃洛格达地区的矮矮的云莓叶子中单宁的鉴定
摘要相关性:深蹲云莓(Rubus chamaemorus)对多种疾病具有足够广泛的治疗作用。同时,它不指药典药用植物,而是作为民间药材使用。在该植物中检测到的单宁(植物单宁)在该植物的其他生物活性物质中占有特殊的地位。它们具有抗炎、抗菌、抗氧化和抗癌活性。此外,它们的特性也适用于预防心血管和代谢疾病。本研究的目的是建立生长在沃洛格达地区的云莓(Cloudberry)叶片中单宁的存在及其定量测定,为该植物药用原料的可能测定提供依据。材料和方法:使用各种化学分析方法测定了生长在沃洛格达地区的深蹲云莓(叶子)原料中单宁的存在和定量含量。研究结果:定性样品证实了所研究植物叶片中单宁的存在(明胶样品),以及其可水解形式的充分存在(铁铵明矾样品);定量(高锰酸钾法)测定单宁含量相对于原料总干重为15.03±1.14%(95%置信区间= 14.12-15.95)。所得结果与其他研究人员的数据一致,单宁相对于干植物总质量的平均相对比例与平均科学结果相关。研究结果表明,生长在沃洛格达地区的红毛茛属药用植物,具有潜在的药用价值。
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