First Report for Pathogenity of Cydia Pomonella Granulovirus and Helicoverpa Armigera Nucleopolyhedrovirus to Indian Meal Moth Plodia Interpunctella Hϋbner (Lepidoptera: Pyralidae) in Vitro

V. Mpalkamou, A. Foteinopoulou, A. Ntoukas, I. Zampara, I. Lagogiannis, S. Mantzoukas
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Abstract

The purpose of this study was to examine the for the first time the effect of Cydia pomonella granulovirus and Helicoverpa armigera nucleopolyhedrovirus on the larvae of the Lepidopteran Plodia interpunctella . L 3 larvae were tested to see whether they were influenced by the infection of the two entomopathogenic viruses Cydia pomonella granulovirus and Helicoverpa armigera nucleopolyhedrovirus. The experiment lasted seven days. The results showed that the effect of the two Baculoviruses was statistically important in relation to the control. The effect of the virus H. armigera nucleopolyhedrovirus was greater than the effect of the virus C. pomonella granulovirus, and this led us to the assumption that the use of entomopathogenic viruses can play an important role in controlling P. interpunctella larvae. The recorded mortality after 7 days was for larvae treated with C. pomonella granulovirus 33.3 to 86.7%, with H. armigera nucleopolyhedrovirus 53.3 to 93.3% and control mortality was 0.7%. This information may appear particularly useful in the future control of the insect’s populations in the warehouse. armigera nucleopolyhedrovirus and C. pomonella granulovirus. Our results also indicate that entomopathogenic viruses can be used as a no specific biopesticide with no range limitation.
波单胞Cydia Pomonella颗粒病毒和棉铃虫helicoppa Armigera核多角体病毒对印度斑间飞蛾Hϋbner(鳞翅目:Pyralidae)体外致病性的首次报道
本研究首次研究了卵圆Cydia pomonella颗粒病毒和棉铃虫Helicoverpa armigera核型多角体病毒对鳞翅目间点Plodia interpunctella幼虫的作用。以3只幼虫为试验对象,观察两种昆虫病原病毒——波单胞Cydia pomonella颗粒病毒和棉铃虫Helicoverpa armigera核型多角体病毒对其感染的影响。实验持续了7天。结果表明,两种杆状病毒的作用与对照组相比具有统计学意义。棉铃虫核多角体病毒的作用大于波单胞细粒病毒的作用,这使我们认为昆虫病原病毒在控制斑间假蝇幼虫方面可能发挥重要作用。pomonella颗粒病毒处理的幼虫7 d死亡率为33.3 ~ 86.7%,棉铃虫核多角体病毒处理的幼虫7 d死亡率为53.3 ~ 93.3%,对照死亡率为0.7%。这一信息在未来对仓库中昆虫种群的控制中可能显得特别有用。棉铃虫核多角体病毒和波蒙胞杆菌颗粒病毒。本研究结果还表明,昆虫病原病毒可以作为一种无特异性的生物农药,没有范围限制。
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