Isolation And Characterization Of Nitrogen Fixing Fungi From Fruit And Vegetable Waste Compost

Rizki Dimas Prasetya, H. Walida, Badrul Ainy Dalimunthe, K. Rizal
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Abstract

Nitrogen fixing fungi are fungi capable of fixing free nitrogen into ammonium or nitrate, so that it can be absorbed by plants. Many species of microbes/fungi have the ability to fix nitrogen, but very few are able to excrete nitrogen in the form of ammonia so that their contribution in providing nitrogen for plants is also still low. This study aims to determine the characteristics and type of nitrogen fixing fungi in fruit and vegetable waste compost. The method used in this research was descriptive method. Identification of nitrogen-fixing fungi was carried out macroscopically by looking at the color of the colonies, the shape of the colonies, and the diameter of the fungal colonies, an then identified by molecularly. There were 4 isolates of nitrogen-fixing fungi capable of forming clear zones on Jensen's media with different morphological characteristics and nitrogen-fixing abilities. The largest diameter of the clear zone was shown in isolate A3 of 3.2 cm which is the fungus Aspergillus niger strain SG1.
果蔬废弃物堆肥中固氮真菌的分离与鉴定
固氮真菌是一种能够将游离氮固定成铵或硝态氮,使其被植物吸收的真菌。许多种类的微生物/真菌都有固定氮的能力,但很少有能够以氨的形式排出氮,因此它们在为植物提供氮方面的贡献仍然很低。本研究旨在确定果蔬废弃物堆肥中固氮真菌的特征和类型。本研究采用描述性方法。固氮真菌的鉴定首先从菌落颜色、菌落形状、菌落直径等方面进行宏观鉴定,然后进行分子鉴定。有4株固氮真菌能在Jensen培养基上形成清晰带,它们具有不同的形态特征和固氮能力。分离物A3的透明区直径最大,为3.2 cm,为黑曲霉菌株SG1。
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