Effect of Ethanol Extract of Camellia Sinensis on Salmonella Typhi Infection in Adult Male Rats

EAS Journal of Pharmacy and Pharmacology Pub Date : 2022-01-31 DOI:10.36349/easjpp.2022.v04i01.002

M. Haider, Z. F. Dewan, Md. Moniruzzaman Khan, M. E. Kabir, M. Shahabuddin, M. Aktar, J. Roy, F. Akter

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Abstract

Background: Typhoid fever, a potentially fatal multisystem illness which is due to infection by Salmonella typhi, is an important health problem in many developing countries including Bangladesh. Investigators from the US Centers for Disease Control and Prevention estimate that there are 21.6 million typhoid cases annually, with the annual incidence varying from 100 to 1000 cases per 100 000 population. Objective: To evaluate the effect of ethanol extract of camellia sinensis on salmonella typhi infection in adult male rats. Materials and Methods: Type of study: This was an experimental study. Duration of study: September 2014-February 2016. Place of study: Department of Pharmacology, BSMMU, Shahbag, Dhaka, Department of Microbiology, BSMMU, Shahbag, Dhaka, Department of Clinical Pathology, BSMMU, Shahbag, Dhaka, Department of Chemistry, Dhaka University, Dhaka, Bangladesh. Materials: Animals: 84 adult male Long Evan’s Norwegian rats were obtained from the animal house of BSMMU. Sample Size Estimation: So, we took six groups and 10 rats in each group. The groups were: Control group (I), Rat infected with S. typhi (II), Rat infected with S. typhi then treated with ethanolic leaf extracts of CAS (III), Rat given ethanolic extracts of CAS only (IV), Rat infected with S. typhi then treated with ciprofloxacin (V), Rat infected with S. typhi then treated with both ciprofloxacin and ethanolic extracts of CAS (VI). Adult male rats were taken for this experiment. The number of rat for each group were 10 (n=10). Methodology: Preparation of ethanol extract of C. sinensis Linn: Home processing Black and Green tea (C. sinensis) was published by the college of Tropical Agriculture and Human resources (2007) which was supported in part by the USDA, agricultural Research Service’s pacific Basin, Agricultural Research center and the Department of Research and Development, County of Hawai’I, Japan. i) Collection of C. sinensis leaves: Harvest 10 kg of fresh tea shoots from ..........

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山茶乙醇提取物对成年雄性大鼠伤寒沙门菌感染的影响

背景:伤寒是由伤寒沙门氏菌感染引起的一种潜在致命的多系统疾病，是包括孟加拉国在内的许多发展中国家的一个重要卫生问题。美国疾病控制和预防中心的研究人员估计，每年有2160万伤寒病例，年发病率从每10万人100例到1000例不等。目的:探讨山茶乙醇提取物对成年雄性大鼠伤寒沙门菌感染的影响。材料与方法:研究类型:本研究为实验性研究。学习时间:2014年9月- 2016年2月。学习地点:达卡BSMMU Shahbag药学系，达卡BSMMU Shahbag微生物学系，达卡BSMMU临床病理学系，达卡Shahbag，达卡大学化学系，孟加拉国达卡。动物:84只成年雄性龙埃文挪威大鼠来自北京医科大学动物舍。因此，我们取6组，每组10只大鼠。各组分别为:对照组(I)、鼠伤寒感染组(II)、鼠伤寒感染组(III)、鼠伤寒感染组(IV)、鼠伤寒感染组(IV)、鼠伤寒感染组(IV)、鼠伤寒感染组(IV)、鼠伤寒感染组(IV)、鼠伤寒感染组(IV)、鼠伤寒感染组(IV)、鼠伤寒感染组(IV)分别用鼠伤寒感染组(IV)、鼠伤寒感染组(IV)分别用鼠伤寒感染组(IV)。每组10只(n=10)。方法:《中华茶醇提取物的制备:家庭加工红茶和绿茶(中华茶)》由热带农业和人力资源学院(2007年)发表，该学院得到了美国农业部、农业研究服务处太平洋盆地、农业研究中心和日本夏威夷县研究与发展部的部分支持。i)收集中华茶树的叶子:从..........上收获10公斤新鲜的茶苗

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EAS Journal of Pharmacy and Pharmacology

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