Integrated analysis of transcriptome, small RNA, and phytohormonal content changes between Artemisia annua Linn. and Nicotiana benthamiana Domin in heterogeneous grafting

Medicinal Plant Biology Pub Date : 1900-01-01 DOI:10.48130/mpb-2023-0002

Boran Dong, Shunuo Li, Xingxing Wang, Shi-yuan Fang, Jinxing Li, Zongyou Lv, Wansheng Chen

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Abstract

Grafting, one of the artificial propagation methods used in plants, is now widely used in flower breeding, vegetable cultivation and stress response research. In order to discover the reason for success of heterograft and information exchange mechanisms, we used Artemisia annua (Aa) as scion and Nicotiana benthamiana (Nb) as rootstock to build a grafting model. After grafting 30 days co-growth, 7,794 DEGs (different expression genes) and 8,214 DEGs were identified in Aa scion and Nb rootstock, respectively. Most of the DEGs belong to defense response and signal transduction in scion and substance metabolism in rootstock, which indicate that the diverging response mechansim of grafted parts. Fifty Nb genes and 20 Aa genes were detected in Aa scion and Nb rootstock, which were regarded as potentially active genes during the grafting process. The most abundant miRNAs are miR159 and miR166 , which may be closely related to their conservation and physiological functions. Besides, miR159 and miR166 could quickly respond to internal change, therefore the two miRNAs should be considered as biomarkers of successful grafting models. And then, as simultaneously screened miRNAs, miR396 and miR6149 could be potential biomarker in Aa and Nb, respectively . Through the analysis of the miRNA-target gene network in differentially expressed miRNAs, transcription factor R2R3-MYB, bHLH , GRAS , GAMYB , SBP-box , MADS-box , IIS in

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综合分析异质嫁接中黄花蒿和多花稔子的转录组、小 RNA 和植物激素含量的变化

嫁接是植物人工繁殖方法之一，目前广泛应用于花卉育种、蔬菜栽培和胁迫反应研究。为了探究异株嫁接成功的原因和信息交流机制，我们以黄花蒿（Aa）为接穗，烟草（Nb）为砧木建立了嫁接模型。嫁接后共同生长30天，在接穗Aa和砧木Nb中分别鉴定出7794个DEGs（不同表达基因）和8214个DEGs。大部分 DEGs 在接穗中属于防御反应和信号转导，在砧木中属于物质代谢，这表明嫁接部位的反应机制不同。在 Aa 接穗和 Nb 砧木中检测到 50 个 Nb 基因和 20 个 Aa 基因，这些基因被认为是嫁接过程中潜在的活性基因。miRNA含量最高的是miR159和miR166，这可能与它们的保护和生理功能密切相关。此外，miR159 和 miR166 能对内部变化做出快速反应，因此这两个 miRNA 应被视为成功嫁接模型的生物标志物。然后，作为同时筛选的 miRNA，miR396 和 miR6149 可分别作为 Aa 和 Nb 的潜在生物标志物。通过对差异表达 miRNA 的 miRNA-靶基因网络的分析，发现转录因子 R2R3-MYB、bHLH、GRAS、GAMYB、SBP-box、MADS-box、IIS 在 Aa 和 Nb 中的表达量分别为 0.5%、0.5%、0.5% 和 0.5%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medicinal Plant Biology

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