The antimicrobial activity of Thymus marshallianus extract against clinical cultures S. aureus and C. albicans

Abstract

Aim to study the antimicrobial activity of the Thymus marshallianus extract against clinical cultures of microorganisms isolated from patients' material. Material and methods. The extract from the herb Th. marshallianus was obtained according to the method previously described and used, since the extraction obtained by this method presented the antimicrobial activity against standard cultures of microorganisms. The antimicrobial activity of extracts was studied in accordance with the Guidelines 4.2.1890-04 for 6 clinical isolates of Staphylococcus aureus and 5 clinical isolates of Candida albicans taken from patients' material (nasopharyngeal smear, blood, wound fluid) in the laboratory of the S. R. Mirovtsev of the Saratov State Medical University. The sectoral seeding was performed on dense nutrient media from test tubes with the colonies count, compared with the control seeding. Results. The antimicrobial activity of Th. marshallianus extract, obtained by the patented method, against clinical cultures of microorganisms was studied for the first time. A pronounced anti-staphylococcal effect of Th. marshallianus extract was established against clinical culture of S. aureus (the minimum inhibitory concentration ranged from 0.78 mg/ml to 0.39 mg/ml). The antimycotic activity was detected only for one culture of C. albicans (minimum inhibitory concentration 25.0 mg/ml).