Analysis of Lipase and Lipid Formation in Adipocytes Inhibitory Capabilities in Kelakai (Stenochlaena palustris) for Obesity Management

Abstract

The objective was to test S. palustris which has high antioxidant activity for bioactivities that may alleviate obesity: lipase inhibitory activity and lipid accumulated in 3T3-L1 adipocytes. Water, 50% methanol and methanol extracts of S. palustris were tested for lipase inhibitory activity and lipid accumulation inhibition in 3T3-L1 adipocytes. For lipase inhibitory activity, the extracts and fractions with highest activity were separated and assayed again. Fractionation through column chromatography was done on the water extract to separate it into water, 50% methanol and methanol fractions. The 50% methanol and methanol fractions from the first column were fractionated again with a different column into water, 20%, 40%, 60%, 80% and methanol fractions. The result was the water extract showed the highest lipase inhibition while the methanol extract showed the highest lipid accumulation inhibition in adipocytes. In conclusion, S. palustris extracts with its lipase inhibitory activity as well as lipid accumulation in adipocytes suppression activity is promising for future research aimed at obesity management.