Tumorcidal Effect of Recombinant Newcastle Disease Virus

Abstract

We have generated GFP expressing recombinant Newcastle Disease Virus (rNDV-GFP) based on a live vac- cine strain which contains a less pathogenic cleavage site of F protein leading to restricted viral growth in vivo. We evalu- ated the rNDV have an effect of oncolytic activity to B16 melanoma cell. The rNDV-GFP infected efficiency to B16 cells and induced cell death. Apoptosis-related genes, Bax and Caspase 3, were elevated in the infected cells. In order to evalu- ate the tumorcidal effect of rNDV on canine tumor cells, two canine tumors such as highly metastasizing canine osteosar- coma (HMPOS) and transitional cell carcinoma (TCC) were infected with rNDV. The rNDV induced cell death to canine tumor cells within 48 hours post infection. These results suggest that rNDV-GFP have a tumorcidal activity to B16 mouse melanoma and canine tumors in vitro.