Evaluation of arbuscular mycorrhizal fungi using 18S rDNA sequence from root of Botrychium ternatum

Bulletin of the Kyushu University Forests Pub Date : 2005-03-27 DOI:10.15017/14856

金東勲, Dong-Hoon Kim, 李晙基, Jun-Ki Lee, 厳安欽, An-Heum Eom, 趙南爽, N. Cho, 須原弘登, Hiroto Suhara, 大賀祥治, S. Oga, キントウクン, リジュンキ, ゲンアンキン, ナムソクチョウ, ヒロトスハラ, ショウジオオガ

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Abstract

The symbiosis with the arbuscular mycorrhiza (AM) was observed in the roots of Botrychium ternatum. The fungal partial 18S ribosomal DNA (rDNA: approximately 450/1800 bp) were amplified from the DNA extracts of fern roots using fungal specific primer, and subcloned to cloning vector (pGEM-T). According to the RFLP pattern of subclone, some clones were selected and their inserts were sequenced. One of them (GLA-006) was predominant in this analysis, however, four (or potentially five) different clone were found in the root of B. ternatum. To infer the phylogenetic relationship, sequences were donated on BLAST Search. According to the result of BLAST Search, neighbor joining analysis was performed. The partial sequencing data of 18S rDNA indicate that these isolates closely related to genus Glomus (AM fungi), especially G. proliferum and G. sinuosum. Three of them were 92-95 % identical to G. proliferum.

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利用黄霉根18S rDNA序列评价丛枝菌根真菌

在白腐菌根中观察到与丛枝菌根(AM)的共生关系。利用真菌特异性引物从蕨类植物根DNA提取液中扩增出真菌18S部分核糖体DNA (rDNA:约450/1800 bp)，并将其亚克隆到克隆载体(pGEM-T)上。根据亚克隆的RFLP模式，选择部分克隆并对其插入片段进行测序。其中一个克隆(GLA-006)在该分析中占主导地位，但在白杨根中发现了4个(或可能5个)不同的克隆。为了推断系统发育关系，在BLAST Search上捐赠了序列。根据BLAST搜索结果，进行邻居连接分析。18S rDNA部分测序数据表明，这些分离株与Glomus属(AM真菌)亲缘关系密切，尤其是G. proliferum和G. sinuosum。其中3个与G. proliferum的同源性为92- 95%。

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Bulletin of the Kyushu University Forests

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