Identification of Simple Sequence Repeats in chloroplast and mitochondrial genome of wheat

A. Mishra, Sakshi Chaudhary, Amrender Kumar, H. Chandrasekharan
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Abstract

Simple Sequence Repeats or microsatellites constitute a significant portion of genomes. However the significance of SSRs in organellar genomes has not been completely understood. The availability of organelle genome sequences allow us to understand the organization of SSRs in their coding and non-coding regions. In the present study, SSRs were identified and categorized in mitochondrial and chloroplast genome of wheat. Based on the study, it was observed that the number of SSRs in non-coding region is more as compared to coding region and frequency of mononucleotides was highest in chloroplast genome of wheat and frequency of tetranucleotides was highest in mitochondrial genome of wheat.
小麦叶绿体和线粒体基因组简单重复序列的鉴定
简单序列重复或微卫星构成了基因组的重要部分。然而,SSRs在细胞器基因组中的意义尚未完全了解。细胞器基因组序列的可用性使我们能够了解SSRs在其编码区和非编码区的组织。本研究在小麦的线粒体和叶绿体基因组中鉴定和分类了SSRs。通过研究发现,小麦非编码区SSRs数量多于编码区,单核苷酸出现频率在叶绿体基因组中最高,四核苷酸出现频率在线粒体基因组中最高。
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