Phenotypic and protein variations among selected cowpea (Vigna unguiculata L. Walp.) varieties

O. Amusa, A. Igbari
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Abstract

The study seeks to evaluate the level of genetic diversity among selected cowpea varieties using both phenotypic traits and seed storage proteins. Twenty cowpea varieties were used in the study. They were planted and phenotypically characterized. Seed proteins were evaluated at maturity. There was a high phenotypic variation observed among the selected cowpea with the evaluated phenotypic traits while a low level of variation was observed with protein evaluation. The variation captured by the phenotype (>90%) was higher as compared to the protein (< 30%) analysis in the study. Three major clusters were generated with the majority of the germplasms in cluster III, regardless of whether the phenotype or protein analysis was used. While the variation observed within the phenotypic traits might have been due to environmental influences, the low variation exhibited using seed storage proteins implies a high level of similarity among cowpea samples. Hence, a more stable marker type should be explored to identify the true level of genetic diversity within cowpea germplasm.
豇豆(Vigna unguiculata L. Walp.)不同品种间的表型和蛋白质变异
本研究旨在利用表型性状和种子贮藏蛋白来评价选定豇豆品种间的遗传多样性水平。研究中使用了20个豇豆品种。将它们种植并进行表型鉴定。在成熟时评估种子蛋白。所选豇豆在评价表型性状方面存在较高的表型变异,而在蛋白质评价方面存在较低的变异水平。表型捕获的变异(>90%)高于研究中的蛋白质分析(< 30%)。无论表型分析还是蛋白分析,聚类III中的大部分种质都产生了3个主要聚类。虽然在表型性状中观察到的变异可能是由于环境影响,但使用种子储存蛋白所表现出的低变异意味着豇豆样品之间的高度相似性。因此,应该探索一种更稳定的标记类型,以确定豇豆种质资源遗传多样性的真实水平。
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