A Novel Lateral Flow Strip Based on DNA-Functionalized Gold Nanoparticles for On-site Detection of Mercury (II) Ions

2019 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN) Pub Date : 2019-05-01 DOI:10.1109/ISOEN.2019.8823368

Jiadi Sun, Ying Gan, Liubing Kong, Shuqi Zhou, T. Liang, Xinyi Wang, H. Wan, Ping Wang

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引用次数: 1

Abstract

Mercury pollution in aquatic environment is becoming increasingly serious, which poses a threat to the ecological environment and human health. Traditional methods are costly and time consuming since they require sophisticated instruments and professional operation. Thus simple and on-site detection of mercury (II) ions (Hg2+) is extremely expected. In this study, we constructed a one-step lateral flow strip that can selectively detect Hg2+ in solution based on the thymine-Hg2+-thymidine (T-Hg2+-T) mismatch principle. Detect probe (poly T20) were designed to bind to compete probe (poly A20) which can compete with Hg2+ to bind detect probe, leading to the color change on the test line. Moreover, a home-made bionic E-eye system was developed as a portable in-situ detection platform to efficiently measure the Hg2+ in 10 minutes by direct photographing. Under optimized experimental conditions, the developed lateral flow strip had a detection limit of 2 ppb and could detect up to 400ppb.

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基于dna功能化金纳米颗粒的新型横向流动条带用于现场检测汞(II)离子

水环境汞污染日益严重，对生态环境和人类健康构成威胁。传统的方法既昂贵又耗时，因为它们需要复杂的仪器和专业的操作。因此，对汞(II)离子(Hg2+)的简单和现场检测是非常期待的。本研究基于胸腺嘧啶-Hg2+-胸腺嘧啶(T-Hg2+-T)错配原理，构建了一种可选择性检测溶液中Hg2+的一步横向流动条。检测探针(poly T20)被设计成与竞争探针(poly A20)结合，而竞争探针(poly A20)可以与Hg2+竞争结合检测探针，导致测试线上颜色变化。自制仿生电子眼系统作为便携式原位检测平台，可在10分钟内通过直接拍摄高效测量Hg2+。在优化的实验条件下，开发的横向流动条检测限为2 ppb，最高可检测400ppb。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2019 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN)

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