Efek Kombinasi Ekstrak Daun Kumis Kucing (Orthosipon aristatus) Dan Daun Sirsak (Annona muricata L.) Terhadap Penurunan Kadar Gula Darah Mencit (Mus musculus)

Abstract

Orthosiphon aristatus and Annona muricate L are traditional medicines used in the treatment of diabetes in Indonesia. Orthosiphon aristatus in the treatment of diabetes and its complications mainly include inhibiting the activity of -amylase and -glucosidase, antioxidant and anti-inflammatory activity, regulating lipid metabolism, increasing insulin secretion, improving insulin resistance, increasing glucose absorption, promoting glycolysis, inhibiting gluconeogenesis, promoting glucagon secretion. -likepeptide-1 (GLP-1) and antiglycation activity. Phenolic acids, flavonoids and triterpenoids may be the main components for the hypoglycemic effect on O. aristatus. The purpose of this study was to determine the effect of the combination of cat whiskers leaf extract (EDK) and soursop leaf extract (EDS) on reducing blood sugar levels in mice (Mus musculus). This study is an experimental study with a randomized control trial pre and post test design using the glucose tolerance method. The test animals used were 15 male mice (Mus musculus) which were divided into 5 groups, namely group 1 control with 1% sodium carboxy methyl salicylic suspension, group 2, comparison group with 0.0065% glibenclamide suspension, group 3 combination EDK 3.5% (0.35 g/kgBW) and EDS 1.05% (105 mg/kgBW), group 4 combined EDK 7% (0.7 g/kgBW) and EDS 0.7% (70 mg/kgBW ), and group 5 a combination of 10.5% EDK (1.05 g/kgBW) and 0.35% EDS (35 mg/kgBW g) which was administered orally with a volume of 0.2 ml/20gBW in mice. The results showed that all treatment groups experienced a decrease in blood sugar levels at 120 minutes but only the combination group of 0.7% EDS and 7% EDK had a greater decrease than Na CMC and was comparable to glibenclamide.