Possible Therapeutic Role of Fermented Deglycyrrhizinized Liquorice Extract on Experimentally Induced Diabetic Keratopathy in Rats. Histological Study

Cytology & Histology Reports Pub Date : 1900-01-01 DOI:10.29011/2688-6421.100007

A. Massoud, F. H. E. Ebiary, Hadwa Ali Abd Al khalek, S. Gawad

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引用次数: 1

Abstract

Introduction: Long-term hyperglycemia has toxic effects on all cells in the body, and its most profound effects on eye tissues are on the cornea and retina. Seventy percent of diabetic patients are complicated by keratopathy, including recurrent corneal epithelial erosions, delayed wound healing, ulcers and edema. Glycyrrhiza glabra; also called Liquorice; is a well-known medicinal plant used in traditional medicine for its pharmacological value. However, excess consumption of Liquorice can lead to hypertension. Therefore, Deglycyrrhizinated Liquorice extract (DGL) is mostly used to avoid the hypertensive side effects of the glycyrrhizin in whole liquorice. Moreover, fermentation of Liquorice can produce excess amounts of amylase and lipase beside a spectrum of flavonoids naturally occurring in liquorice, hence the newly innovated Fermented Deglycyrrhizinized Liquorice (FDGL) was used in this study. Aim: In the present study we aimed to investigate the potential therapeutic effects of FDGL, as a natural product, on experimentally induced diabetic keratopathy in rats. Materials and Methods: Forty adult male albino rats were divided into four groups. Group I: Control animals, which were divided into citrate buffer treated and FDGL treated subgroups (10 rats each). Group II (diabetic) and Group III (diabetic and FDGL treated), 10 rats each. At the end of experiment, the rats were sacrificed and the corneas of different groups were processed for light and electron microscopic examination. The thickness of corneal layers was measured by image analyser and statistical analysis was done. Results: Light microscopic examination of the cornea of diabetic group showed numerous cells with darkly stained nuclei together with focal sloughing of the surface epithelial layers. Also there was a significant decrease of mean corneal thickness, comparable to the control group. Transmission electron microscopic examination of the cornea of the diabetic group revealed ill-defined hemidesmosomal junctions with interruption of the desmosomal junctions. The stroma showed focal loss of collagen fibrils. Diabetic and FDGL treated group (Group III) showed amelioration of the effects of diabetes on the structure of the cornea. Conclusion: The data obtained from the present study revealed that FDGL has a potential ameliorating effect on diabetes mellitus induced structural changes in the cornea. Citation: Massoud AMA, El Ebiary FH, Alkhalek HAA, Gawad SA (2019) Possible Therapeutic Role of Fermented Deglycyrrhizinized Liquorice Extract on Experimentally Induced Diabetic Keratopathy in Rats. Histological Study. Cytol Histol Rep 2: 107. DOI: 10.29011/CHR-107.100007 2 Volume 2; Issue 01

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甘草去甘草酸发酵提取物对实验性糖尿病大鼠角膜病变的治疗作用。组织学研究

导读:长期高血糖对体内所有细胞都有毒性作用，对眼部组织影响最深的是角膜和视网膜。70%的糖尿病患者并发角膜病变，包括复发性角膜上皮糜烂、伤口愈合延迟、溃疡和水肿。甘草glabra;也叫甘草;是一种著名的药用植物，因其药理价值而被用于传统医学。然而，过量食用甘草会导致高血压。因此，为了避免全甘草中甘草酸苷对高血压的副作用，多使用去甘草酸甘草提取物(DGL)。此外，甘草发酵可以产生过量的淀粉酶和脂肪酶，以及甘草中天然存在的黄酮类化合物，因此本研究采用了新发明的发酵脱甘草酸甘草(FDGL)。目的:探讨天然产物FDGL对实验性大鼠糖尿病性角膜病变的潜在治疗作用。材料与方法:40只成年雄性白化大鼠分为4组。第一组:对照动物，分为枸橼酸缓冲液组和FDGL组，每组10只。II组(糖尿病组)和III组(糖尿病组和FDGL组)，各10只。实验结束后处死大鼠，取各组角膜进行光镜、电镜检查。用图像分析仪测量角膜各层厚度，并进行统计分析。结果:糖尿病组角膜光镜下可见大量细胞，细胞核深染，表面上皮层局灶性脱落。与对照组相比，平均角膜厚度也有显著下降。糖尿病组角膜透射电镜检查显示半间粒连接不清，间粒连接中断。基质中胶原原纤维局部丢失。糖尿病和FDGL治疗组(III组)显示糖尿病对角膜结构的影响有所改善。结论:FDGL对糖尿病引起的角膜结构改变具有潜在的改善作用。引用本文:Massoud AMA, El Ebiary FH, Alkhalek HAA, Gawad SA(2019)发酵脱甘草酸甘草提取物对实验性糖尿病大鼠角膜病变的可能治疗作用。组织学研究。细胞组织学报告2:107。DOI: 10.29011/ chrr -107.100007 2卷2;问题1

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Cytology & Histology Reports

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