Droplet bisulfite conversion platform for epigenetic cancer biomarker detection

2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII) Pub Date : 2013-10-10 DOI:10.1109/TRANSDUCERS.2013.6627235

D. J. Shin, A. Stark, T. H. Wang

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Abstract

We report an integrated digital microfluidic platform for methylation biological samples. Recent advances in epigenetic studies highlight the importance of DNA methylation in the biology of cancer. The epigenetic signature of promoter regions in the genes ADAMTS1 and BNC1 has been shown to have strong correlation with the early stages of pancreatic carcinogenesis [1]. Most analytical techniques for studying methylation require pre-processing of sample DNA via bisulfite conversion. However, repetitive addition and removal of liquid reagents using conventional tubes and pipettes present a technical hurdle for microliter-scale reagents, as well as a high risk for contamination and human error. Previously, we reported a magnetically actuated platform for integrated DNA extraction and amplification from crude biological samples [2]. In this work, we demonstrate a self-contained, magnetically-actuated droplet cartridge for bisulfite treatment of genomic DNA with enhanced performance compared to the tube-based process.

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亚硫酸氢盐液滴转化平台用于表观遗传癌症生物标志物检测

我们报告了一个集成的数字微流控平台甲基化生物样品。表观遗传学研究的最新进展强调了DNA甲基化在癌症生物学中的重要性。ADAMTS1和BNC1基因启动子区域的表观遗传特征已被证明与胰腺癌的早期发生有很强的相关性[1]。大多数研究甲基化的分析技术需要通过亚硫酸盐转化对样品DNA进行预处理。然而，使用传统的管和移液器重复添加和移除液体试剂对微升规模的试剂来说是一个技术障碍，并且存在污染和人为错误的高风险。此前，我们报道了一种用于从粗生物样品中提取和扩增DNA的磁驱动平台[2]。在这项工作中，我们展示了一种独立的、磁驱动的亚硫酸氢盐处理基因组DNA的液滴盒，与基于管的工艺相比，它具有更高的性能。

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2013 Transducers & Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII)

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