Changes in the antioxidant system under the action of radiation in the long term period

Abstract

: The aim of the study was to study changes in the antioxidant system of immunocompetent organs and cells when exposed to sublethal doses of gamma radiation in the long term. The study was conducted on 40 male rats divided into 4 groups: group I - intact, group II – research after seven days of irradiation, group III – research after thirty days of irradiation and group IV – research after ninety days of irradiation. On the 7th and 30th days of the study, after high-dose γ -radiation in the studied organs, a sharp decrease in the activity of enzymes of the antioxidant system was noted, and on the 90th day, i.e. in the remote period of irradiation, inhibition of the activity of a greater degree of the catalase enzyme remains. title not content, it not contain bibliographic it reflect the summary of the article, preserving the structure of the article - introduction/ problem statement /goals/ history, research methods, results /discussion, conclusion). Keywords (6-8 words/word combination. Keywords should reflect the main content of the article, use terms from the as well as terms that define and include other important concepts that make it easier and more convenient to Abstract: Efficient infection of Nicotiana benthamiana plants with wild type Tomato bushy stunt virus (TBSV) is influenced by expression of protein P19, which is a potent RNAi suppressor. The capsid protein (СР) P41 is required for virion formation and facilitates long distance movement of the virus. Along with RNAi suppression, P19 protein is involved in the development of severe disease symptoms in N. benthamiana and elicitation of Hypersensitive Response (HR) in tobacco. Our results show that wild type TBSV infection of Solanum lycopersicum (cv. Money maker) triggers resistance to the virus. Despite detectable accumulation levels of P19 protein in leaf and root tissues, the infection was not accompanied with obvious disease symptoms. Contrastingly, inoculation with TBSV mutant, lacking capsid protein P41 demonstrated susceptibility to TBSV. Moreover, Chl-FI analysis of plants infected with virus exhibited significant changes in metabolism. Our data suggests that in response to CP expression tomato plants have evolved defense mechanisms to resist viral infection.