PENGARUH KOSENTRASI NAA DAN TDZ (THIDIAZURON) TERHADAP ORGANOGENESIS KALUS KENCUR (Kaempferia galanga L.)

Abstract

The production of kencur (Kaempferia galanga L.) with tissue culture is an alternative in kencur plant cultivation as a provider of seeds. In tissue culture, growth regulators can affect the formation of plant organs (organogenesis). The combination of growth regulators NAA and TDZ was used in this study to induce callus kencur organogenesis. The purpose of this research was to determine the effect of giving NAA and TDZ on the organogenesis of kencur callus, as well as the optimal combination of the two in the organogenesis of kencur callus. The design used was factorial RAL with two factors: NAA (0; 0.5 and 1 ppm) and TDZ (0; 2 and 4 ppm). Observation variables included number of shoots, shoot length, number of leaves, leaf length, number of roots, root length, fresh weight, and the first time shoots appeared. The results showed that the single NAA treatment affected the number of shoots, number of leaves, number of roots, root length, and fresh weight. In comparison, the single TDZ treatment affected shoot length, leaf length, number of roots, root length, and fresh weight. The optimal treatment combination was 1 ppm NAA and 2 ppm TDZ (N1T2), which gave the highest number of shoots. Higher concentrations of cytokinins can stimulate shoot growth.