Quarantine and Antibiotic Susceptibility of Enterobacteriaceae Strains and other Gram-Negative Bacteria in Dairy Sweetmeat Milk (Doodh) Peda

Abstract

The present study aimed to assess the prevalence and isolation of Enterobacteriaceae strains and other gram-negative bacteria of milk (doodh) peda-a traditional milk product of India. Bacteria of the family Enterobacteriaceae are the indicator organisms that provide evidence of poor hygiene, inadequate processing or post-process contamination of foods. A total of 25 peda samples were collected from different places in the Mysuru district to evaluate the prevalence of Enterobacteriaceae. Seventeen gram-negative fermentative bacteria of clinical significance were detected and isolated. API 20E biochemical identification syatem and other associated biochemical tests are used as a supportive tool to identify gram-positive and gram-negative bacteria. Our outcomes indicate that milk peda contaminated with the Enterobacteriaceae like Enterobacter cloacae, Yersinia bercovieri, Yersinia rohdei, Raoutlella terigena, Acinetobacter lwoffii, Pantoea agglomerans PA2, Klebsiella pneumoniae subsp pneumoniae, Leclericia adecarboxylata, Photorhabdus nematophilus, Enterobacter gergoviae, Acinetobacter baumannii/calcoacetius, Cronobacter sakazaki (Enterobacter sakazaki). Further, the antimicrobial resistance of these Enterobacteriaceae groups was also investigated against 20 antibiotics by the disc diffusion assay method. All isolates revealed susceptibility to the fluoroquinolones and aminoglycosides. But resistance to the nitrofurantoin (70.59 %), augmentin (52.94%), and cefpodoxime (47.06%) antibiotics. Our finding was the first report of the prevalence and detection of E. gergoviae in a food sample (milk peda) and examined for antibiotic resistance.