EFFECT OF VASCULAR ENDOTHELIAL GROWTH FACTOR ON SPERMATOGENESIS AFTER 2 GY ELECTRON IRRADIATION

Abstract

Platelet-rich plasma (PRP) influences tissue repair, stimulating the proliferation and differentiation of progenitor cells. The aim of the study was to evaluate the effect of vascular endothelial growth factor (VEGF) on spermatogenesis after 2 Gy electron irradiation. Materials and Methods. Light microscopy and immunohistochemical reactions with antibodies to VEGF were used to examine testes of 5 groups of experimental animals (Group 1 – control (n=10), Group 2 – 2IR (n=35), Group 3 – 2IR+LP-PRP+IGF-1 (n=30), Group 4 – 2IR+LP-PRP (n=30), Group 5 – LP-PRP (n=30)). Results. After irradiation, a decrease in the number of VEGF-positive cells was observed, which was restored under PRP administration. Conclusion. 2 Gy electron irradiation of the testes leads to a decrease in VEGF-positive cells. PRP administration, containing vascular endothelial growth factor, enhances reparative processes and restores spermatogenesis.