Effect of Pulsed Electric Fields on the Recovery of Antioxidant Protein Extracts from Fish Side Streams

Foods 2021 Pub Date : 2021-10-13 DOI:10.3390/foods2021-10962
F. J. Martí-Quijal, F. Barba, Francesc Ramon-Mascarell
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Abstract

: Fish side streams represent between 20–80% of the weight of fish, and their disposal represents an environmental and economic problem. These side streams contain proteins and antioxidant compounds that give them added value. In order to valorise these side streams, an extraction assisted by pulsed electric fields (PEF) was carried out to obtain proteins and compounds with antioxidant activity. An extraction for the same time but without PEF pre-treatment was used as a control. The side streams used were head, skin, viscera and backbone from sea bass. The antioxidant capacity was measured by two complementary methods, TEAC and ORAC. The results showed that in the case of head and skin, treatment with PEF notably improved protein extraction by 37.7 and 37.8%, respectively. However, for viscera, the best values were achieved with the control extraction. Finally, backbone did not show significant differences in protein recovery between PEF-assisted extraction and control extraction. Regarding antioxidant capacity, the results followed the same trend as for proteins. For head and skin, the pre-treatment with PEF increased the extraction of antioxidant compounds, improving the TEAC values by 21.74 and 29.11%, respectively. On the other hand, ORAC results increased 22.11% for head extracts and 40.93% for skin extracts. In the extracts obtained from the viscera, the TEAC and ORAC values were better in the control sample, being 21.43% and 24.82% higher, respectively. The antioxidant compounds present in the samples may be bioactive peptides, but a more exhaustive analysis using chromatographic techniques would be necessary to complete the information. Therefore, it has been seen that PEF-assisted extraction can be a good strategy to increase the recovery of proteins and antioxidant compounds from fish side streams such as head or skin, but not for viscera and backbone.
脉冲电场对鱼侧溪中抗氧化蛋白提取物回收的影响
鱼类侧流占鱼类重量的20-80%,它们的处理是一个环境和经济问题。这些侧流含有蛋白质和抗氧化化合物,使它们具有附加价值。为了使这些侧流增值,在脉冲电场(PEF)的辅助下进行了提取,以获得具有抗氧化活性的蛋白质和化合物。以同一时间未进行PEF预处理的提取为对照。侧流使用的头部,皮肤,内脏和脊骨从海鲈鱼。采用TEAC和ORAC两种互补法测定其抗氧化能力。结果表明,对于头部和皮肤,PEF处理显著提高了蛋白提取率,分别提高了37.7%和37.8%。而对于脏器,以对照提取为最佳。最后,骨架在pef辅助提取和对照提取之间的蛋白质回收率没有显着差异。在抗氧化能力方面,结果与蛋白质的趋势相同。对于头部和皮肤,PEF预处理增加了抗氧化化合物的提取,TEAC值分别提高了21.74%和29.11%。另一方面,头部提取物ORAC提高22.11%,皮肤提取物ORAC提高40.93%。在脏器提取物中,对照样品的TEAC和ORAC值分别高出21.43%和24.82%。样品中存在的抗氧化化合物可能是生物活性肽,但使用色谱技术进行更详尽的分析将是必要的,以完成信息。因此,已经看到pef辅助提取可以是一个很好的策略,以增加蛋白质和抗氧化化合物的回收从鱼的侧流,如头或皮肤,但不是内脏和脊柱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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