Aktivitas Gel Kolagen Sisik Ikan Bandeng (Chanos chanos) Kombinasi Ekstrak Etanol Daun Bidara (Ziziphus jujuba) Terhadap Penyembuhan Luka Bakar Pada Kelinci (Oryctolagus cuniculus)

Abstract

This study aims to formulate scale collagen of milkfish (Chanos chanos) combined with ethanol extract of bidara leaves (Ziziphus jujuba) in the form of a gel preparation and see its stability and activity by testing the effect of healing burns on rabbits (Oryctolagus cuniculus). This combination is necessary because the nature of the collagen can regenerate cells and the active substance in bidara leaf extract is also efficacious as an anti-inflammatory so that the combination of Both are able to accelerate the healing of burns. The research was carried out using an experimental method by making 3 formulations of gel preparations with different concentrations of 0.3%, 0.6%, and 0.9% dry collagen, while the concentration of the dried Chinese bidara leaf extract was the same 5% for each formula, with hydroxyl variations. ethyl cellulose 1%, 1.25%, and 1.5%. Furthermore, the physical stability test of the gel before and after accelerated storage was carried out including organoleptic tests, homogeneity, pH, viscosity, and syneresis tests were then carried out in vitro tests to see the activity of milkfish scale collagen (Chanos chanos) combined with ethanol extract of bidara leaves (Ziziphus jujuba) on the healing of burns in rabbits. From the research, it was found that the three gel formulas had good gel physical stability before and after storage. In the homogeneity and organoleptic test, it looks homogeneous, transparent brownish yellow with a characteristic odor. pH value already according to the SNI standard (4.57.8), the viscosity is in the range (2000-50.000 cps). The highest syneresis test was 9.35% in formula 1. In the burn healing activity test which was observed for 20 days, the most effective decrease in burn diameter was in formula 3 with a percentage decrease of 89.5% (P < 0,05)