[Effect of ultraviolet light on the UV/VIS absorption spectrum of components of Eagle (MEM) cell culture medium].

Abstract

The impact of ultraviolet light on uv/vis absorption spectra of selected individual components of the cell breeding medium according to Eagle (MEM) was investigated. The strongest alterations of light absorption were detected in L-phenylalanine, L-tyrosine and L-tryptophan. Thus, the absorption behaviour of the Eagle (MEM) medium changed post radiation may be attributed to spectrophotometric alterations of absorption in aromatic amino acids. The results are discussed with regard to the effect on the surface charge of erythrocytes.