ISOLATION AND CHARACTERIZATION OF PLANT GROWTH-PROMOTING BACTERIA FROM DIFFERENT RHIZOSPHERIC SOILS

THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS Pub Date : 2022-12-31 DOI:10.52700/jmmg.v3i3.94

Rimsha Yasin, Sunbul Rasheed, M. Cheema, A. Amin

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Abstract

The production of indole acetic acid (IAA) is a significant property of rhizospheric bacteria to stimulate and promote plant growth. In this investigation, indole acetic acid-producing bacteria from the rhizosphere of five distinct plants (Wheat, Gulchen, Rose, Lady Finger, and Bouginvella) were isolated and characterized. Bacterial strains were isolated and identified by morphological characterization. Out of 30 bacterial isolates, 5 isolates were selected as efficient producers of Indole acetic acid. These selected bacterial isolates were subjected to several biochemical tests for identification at the genus level. After the determination of bacterial genus, antibiotic sensitivity test was performed to check the resistance pattern of each bacterial isolate against antibiotics. Selected bacterial isolates were characterized by using molecular techniques. DNA of five bacterial isolates was extracted by WizPrepTMg DNA mini kit and amplification of 16S rRNA gene was done by PCR. Amplification of PCR product was confirmed by agarose gel electrophoresis. From biochemical tests, Klebsiella spp., E. coli, and Bacillus sp. tested positive. Klebsiella spp. showed 87, 88, 81, 88, 91, 100, 90, and 87% resistance against enrofloxacin, gentamycin, penicillin, ampicillin, chlotetracyclin, tylosine, oxytetracycline, and amoxicillin, respectively. E. coli showed 80, 88, 79, 84, 79, 86, 82, and 89% against enrofloxacin, gentamycin, penicillin, ampicillin, chlotetracyclin, tylosine, oxytetracycline, and amoxicillin respectively whereas 87, 91, 100, 100, 93, 100, 89 and 100% resistance was shown by Bacillus sp. against enrofloxacin, gentamycin, penicillin, ampicillin, chlotetracyclin, tylosine, oxytetracycline, and amoxicillin, respectively. All these selected bacterial isolates were found positive for the 16S rRNA gene and characterized as Klebsiella pneumonia RY6, Klebsiella oxytoca strain RY8, Klebsiella oxytoca RY10, Escherichia coli RY20, and Bacillus tequilensis RY23. It is concluded that the property of synthesizing IAA is considered an effective tool for the isolation and characterization of bacteria that have a role in promoting plant growth.

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不同根际土壤植物促生细菌的分离与鉴定

吲哚乙酸(IAA)的产生是根际细菌刺激和促进植物生长的重要特性。本研究从5种不同的植物(小麦、古尔琴、玫瑰、Lady Finger和布金菊)根际分离并鉴定了产吲哚乙酸的细菌。分离菌株并进行形态鉴定。从30株分离菌中筛选出5株为吲哚乙酸的高效产菌。这些选定的细菌分离株进行了几次生化试验，以在属水平进行鉴定。细菌属确定后，进行药敏试验，检查各分离菌对抗生素的耐药模式。所选细菌分离物采用分子技术进行鉴定。采用WizPrepTMg DNA迷你试剂盒提取5株细菌的DNA，用PCR扩增16S rRNA基因。琼脂糖凝胶电泳证实了PCR产物的扩增。生化检测显示克雷伯氏菌、大肠杆菌和芽孢杆菌呈阳性。克雷伯氏菌对恩诺沙星、庆大霉素、青霉素、氨苄西林、绿霉素、泰洛氨酸、土霉素和阿莫西林的耐药率分别为87%、88%、81%、88%、91%、100%、90%和87%。大肠杆菌对恩诺沙星、庆大霉素、青霉素、氨苄西林、绿霉素、tylosine、土霉素和阿莫西林的耐药率分别为80%、88%、79%、84%、79%、86%、82%和89%，而芽孢杆菌对恩诺沙星、庆大霉素、青霉素、氨苄西林、绿霉素、tylosine、土霉素和阿莫西林的耐药率分别为87%、91%、100%、100%、93%、100%、89%和100%。所选菌株均为16S rRNA基因阳性，鉴定为肺炎克雷伯菌RY6、氧化克雷伯菌RY8、氧化克雷伯菌RY10、大肠埃希菌RY20和龙舌兰芽孢杆菌RY23。综上所述，合成IAA的特性可作为分离和鉴定具有促进植物生长作用细菌的有效工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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THE JOURNAL OF MICROBIOLOGY AND MOLECULAR GENETICS

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