LAMBEDA PHAGE

Abstract

Background: Recognition of bacteriophages in many aspects plays an important role such as controlling the number and variation of bacteria and participation in horizontal gene transfer, which is an important process in bacterial evolution. Bacteriophages use small proteins to take over the host molecular machinery and thus interfere with central metabolic processes in infected bacteria. In general, phages inhibit or reverse host transcription to transcribe their genome. Mechanical and structural studies of phage host transcription may lead to the development of new antibacterial agents. Result: The result shows that phage vB_Eco4M-7 must be a lytic virus. This was confirmed by monitoring faglitic development by a one-step growth test. In addition, phage of relatively small uniform plaques (1 mm in diameter) occur without lysogenesis. Electron microscopic analysis showed that vB_Eco4M-7 belongs to the family Myoviridae. Based on mass spectrometric analysis, including the fragmentation pattern of unique peptides, 33 vB_Eco4M-7 phage proteins are assigned to the annotated reading frames. The results indicate that the phage studied is a potential candidate for phage treatment and / or food protection against E. coli, which produce Shiga toxin, as most of these strains belong to the O157 serotype. Conclusion: In general, phages inhibit or reverse host transcription to transcribe their genome. Mechanical and structural studies of phage host transcription may lead to the development of new antibacterial agents. The result shows that phage vB_Eco4M-7 must be a lytic virus. This was confirmed by monitoring faglitic development by a one-step growth test.