Effect of Ethanol Extract of Robusta Coffee (Coffea canephora) on the Function of the Liver and Kidney of Wistar Rat

Abstract

—Phytochemical tests of coffee show that coffee contains antioxidant compounds, which play a role in the healing process of disease. However, it is necessary to know the safe dosage of coffee extract used in treating diseases including its effect on important organs such as the liver and kidneys. The purpose of this study was to determine the effect of ethanol extract of Robusta coffee beans on the plasma of AST, ALT, urea, and creatinine of Wistar rats. This study used white male Wistar rats which were divided into Group K (control), Group P1 was given ethanol extract of Robusta coffee beans (EECB) with a dose of 625 mg/Kg BW, Group P2 with a dose of 1250 mg/Kg BW. Group P3 dose 2500 mg/Kg BW, Group P4 dose 5000 mg/Kg BW. Examination of ALT, AST, urea, and creatinine by spectrophotometry. The results showed an increase in the mean levels of ALT, AST, and urea rat plasma in the P1-P4 group, and there was a significant difference using the ANOVA test with a p-value <0.05. Creatinine levels did not show any difference. PostHoc Tukey test, AST levels significantly increased in the P1-P4 group, ALT increased in the P3 and P4 groups, while urea in the P4 group. The ethanol extract of Robusta coffee beans increased AST levels at a dose of 625-5000 mg/Kg BW, increased plasma ALT levels at a dose of 2500-5000 mg /Kg BW, and increased urea levels at a dose of 5000 mg/Kg BW.