Synthesis of Procaine-Based Carbon Dots for Bioimaging and Effective Inhibition of Human Colon Cancer Cell Proliferation

Xiaoming Zhao, Tianyang Qi, Mingxi Yang, Wenjing Zhang, Chenfei Kong, Miao Hao, Yuqian Wang, Hao Zhang, Bai Yang, Jie Yang, Jinlan Jiang
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Abstract

The photoluminescent carbon-based nanomaterials have been utilized in various fields, such as biological detection, drug delivery, and bioimaging; however, their specific biological functions have been rarely studied. Procaine (PCA), a local anesthetic FDA approved drug, has been reported as a tumor inhibitor. In our study, we utilized this nontoxic clinical drug to synthesize a type of novel carbon dots, termed P-dots, via hydrothermal synthesis. Imaging data demonstrated that the as-prepared P-dots have a narrow size distribution within 3.3 nm and excellent luminescent properties suitable for bioimaging. Compared with PCA, the P-dots exhibited stronger inhibition efficiency of colon cancer cell (DLD1) proliferation, and similar low toxicity in vitro and in vivo. The anti-cancer activity of the P-dots was suggested to be associated with the caspase-3 related cell apoptosis. Taken together, our results suggest that synthesisof carbon dots from PCA have dual functions of bioimaging and anticancer effect. It can be speculated that these features of P-dots will enable them to be utilized as safe and effective nanomedicines for clinical usage.
普鲁卡因碳点的合成及其对结肠癌细胞增殖的抑制作用
光致发光碳基纳米材料已广泛应用于生物检测、药物传递和生物成像等领域;然而,其具体的生物学功能却很少被研究。普鲁卡因(PCA)是FDA批准的一种局部麻醉剂,据报道是一种肿瘤抑制剂。在我们的研究中,我们利用这种无毒的临床药物,通过水热合成法合成了一种新型的碳点,称为p点。成像数据表明,制备的p点在3.3 nm范围内具有狭窄的尺寸分布,具有良好的发光性能,适合生物成像。与PCA相比,P-dots对结肠癌细胞(DLD1)的增殖具有更强的抑制作用,并且在体外和体内具有相似的低毒性。P-dots的抗癌活性可能与caspase-3相关的细胞凋亡有关。综上所述,我们的研究结果表明,由PCA合成的碳点具有生物成像和抗癌的双重功能。可以推测,P-dots的这些特性将使其能够作为安全有效的纳米药物用于临床。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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