Automated Quantification and Simulation of Hippocampal Images Treated with Kainic Acid from Rat Brain Slices

Abstract

In the animal models, Kainic acid (KA) has been commonly used in inducing epilepsy especially Temporal lobe epilepsy (TLE) which is more common in adult human. Following administration of KA, there are several biochemical, mitochondrial and neuronal dysfunctional changes occurred in many brain regions including hippocampus and its sub region namely CA1, CA3 and in the dentate gyrus (DG). some dysfunctional changes can be studied and quantified by histopathological methods. This study aimed to quantify the microscopic images stained with hematoxylin and eosin (H&E) after treated with KA in different concentrations. Following analysis, the neurodegenerative changes induced by higher concentrations of KA showed significantly different in its Feret diameter, shape descriptors and integrated density compared to lower dose of KA. In order to optimize the KA induced neurodegeneration a model with simulation study is necessary to improve the KA doses and treatment duration, which might be helpful to save the time.