Identification of the Colletotrichum species associated with mango diseases
and a universal LAMP detection method for C. gloeosporioides species complex
Yukako Hattori, C. Nakashima, Shunsuke Kitabata, Kosuke Naito, A. Hieno, L. V. Alvarez, Keiichiro Motohashi
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引用次数: 1
Abstract
Abstract: The Colletotrichum gloeosporioides species
complex contains plant pathogens linked to Anthracnose
diseases afflicting various crops. In this study, we
designed a loop-mediated isothermal amplification
assay (LAMP) primer set based on calmodulin
gene coding region sequences from taxonomically
authorized isolates of species from this complex to
rapidly detect the presence of fungi associated with
Anthracnose diseases. This test can be employed at
any point between cultivation and sale. Moreover, we
examined the specificity and detectable range of this
primer set using isolates selected from species of the
genus Colletotrichum. This test was able to specifically
detect members of the C. gloeosporioides species
complex, including C. gloeosporioides, C. aotearoa, C.
fructicola, C. horii, C. kahawae, C. musae, C. siamense,
C. theobromicola, and C. tropicale.
Key Words: Anthracnose, diagnosis, phylogeny, plant
disease
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