Diversity Analysis of Rhizosphere Soil Microbial Population Structure and Screening and Identification of Dominant Salt-Tolerant Strains of JunCao"Oasis No. 1"

Abstract

Purpose: As a newly cultivated grass species widely used in saline-alkali soil treatment, Juncao"Oasis No.1" can effectively enhance the microbial population abundance in saline-alkali soil. It has a good effect on improving saline-alkali soil.Methods: In order to further study the microbial community in the rhizosphere soil of Juncao"Oasis No.1" , the dominant strains with important ecological value were screened out. In this paper, soil samples were collected from saline-alkali soil planted with Juncao"Oasis No. 1" and unplanted Juncao"Oasis No. 1", and the microbial populations were sequenced by 16SrDNA high-throughput sequencing, in order to identify the difference of microbial population, the dominant bacteria in rhizosphere soil of Juncao"Oasis No. 1" was selected, and the dominant bacteria with important ecological value were isolated and cultured and identified by Colony PCR.Results: The results showed that Pseudomonas fluorescens and Bacillus thuringiensis. were the dominant strains with important ecological value in rhizosphere soil of Juncao"Oasis No.1" . After isolation and culture, Colony PCR identification showed that the dominant strain was successfully isolated and cultured.Conclusions: In this paper, the dominant strains with important ecological value in rhizosphere soil of Juncao" Oasis No. 1" were screened and cultured, which provided theoretical basis for the improvement of saline-alkali soil by using Juncao"Oasis No. 1", it is of great guiding significance.