PCR-analysis of photoperiod sensitivity genes in bread wheat varieties from Bilatserkovska experimental breeding station

Abstract

The aim. Determination of alleles of the photoperiod sensitivity genes Ppd-1 in 16 winter wheat varieties of the Bilatserkovska Experimental Breeding Station and identification of the haplotypes of Ppd-D1 gene according to the accepted classification. Methods. DNA isolation, allele-specific and nested PCR, electrophoresis in agarose and polyacrylamide gels, determination of the significant differences in the time of heading. Results. Plants of the varieties Vodohrai bilotserkivs’kiy; Bilotserkivs’ka napivkarlykova, Olesia, Perlyna lisostepu, Elehiia, Yasochka, Lybid’, Tsarivna, Lisova pisnia, Romantyka, Vidrada, Schedra nyva, Charodiika bilotsekivs’ka, Russa, Driada 1 have the genotype PpdA1b Ppd-B1b Ppd-D1a and belong to the VII haplotype of the Ppd-D1 gene. The genotype of the variety Legenda bilotsekivs’ka is characterized by the presence of the alleles Ppd-A1b Ppd-B1b Ppd-D1b and belongs to the IV haplotype of the Ppd-D1 gene. The significant differences (P = 0.01) in the time of heading was detected only between the varieties Russa and Legenda bilotsekivs’ka and was 12.4 days. Conclusions. Most BEBS varieties are characterized by genotype — PpdA1b Ppd-B1b Ppd-D1a, in which the allele Ppd-D1a determines insensitivity to the photoperiod and leads to early heading. Only variety Legenda bilotsekivs’ka is the carrier of the recessive allele of Ppd-D1 gene, belongs to the IV haplotype, has the latest time of heading. Keywords: Ppd-A1, Ppd-B1, Ppd-D1 genes, winter wheat, PCR analysis, photoperiod sensitivity.