正常ヒト血液細胞におけるリゾチームの局在: Immumogold staimimg methodによる超微形態学的観察

Abstract

Ultrastrutural localization of lysozyme in human blood cells which were obtained from 10 nonmal volunteers was investigated by electron microscopy applying an immunogold staining method.The presence of lysozyme was demonstrated as the deposition of gold particles by this staining. Lysozyme was detected in neutrophils, monocytes, eosinophils, reticlum cells and mast cells. On the other hand, no lysozyme was detected in basophils, megakaryocytes, platelets, lymphocytes, plasma cells and erythroid cells.Generally, lysozyme was preferentially contained in the granules of lysozyme-positive blood cells. In contrast, no lysozyme was detected in the nucleus, perinuclear space, rough endoplasmic reticulum and mitochondria. In neutrophils, both primary and secondary granules contained abundant lysozyme. In monocytes, the granules were remarkably positive for lysozyme. In eosinophils, the granules were positive for lysozyme, but crystalloids negative. In some reticulum cells, phagolysosomes contained small amount of lysozyme. In some mast cells, abundant lysozyme was present in the granules.Electron microscopic immunogold staining is a useful method to know the exact localization of lysozyme in blood cells.