Influence of high-power EM-videopulse on insulated cells

2000 10th International Crimean Microwave Conference. "Microwave and Telecommunication Technology". Conference Proceedings (IEEE Cat. No.00EX415) Pub Date : 2000-09-11 DOI:10.1109/CRMICO.2000.1255849

N. Gaponenko, N. Dikij, Y. Lonin, E. Medvedeva, N. I. Onishchenko

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Abstract

The influence of a short high-power electromagnetic videopulse on a suspension of hepatic cells of 9-weeks human embryo has been investigated. Such pulse was generated by an electron beam of the accelerator "Temp-A" with energy 0.6 MeV, current 4 kA, and time duration 40 nsec falling to rod antenna of 3 m length. The output power was 100 MW. The samples were placed perpendicular to the antenna at 30 cm distance from its center. The free-radical state in the system "hepatocite+hypochlorite" has been investigated using the quantum-metrical set with photo-electronic amplifier FEU-140 in a thermostat box at 37/spl deg/C. The appearance of radical fragments in this system was registered by means of 630 nm spectral line of generated single oxygen. The estimation of the cell viability before and after irradiation was performed using the method of supravital staining by methylene dark blue. It was shown that the influence of video-pulse treatment decreases the percentage of the viability of the hepatocites and activates the free-radical processes and correspondingly enhances the single oxygen generation. The dependence of these processes on the video-pulse power was determined.

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大功率电磁视频脉冲对绝缘细胞的影响

本文研究了短功率电磁视频脉冲对9周人胚胎肝细胞悬浮液的影响。该脉冲由能量0.6 MeV、电流4 kA、持续时间40 nsec的加速器“Temp-A”电子束落在3 m长的棒天线上产生。输出功率为100mw。样品与天线垂直放置，距离天线中心30厘米。在37℃恒温箱中，利用带光电放大器FEU-140的量子测量装置研究了“肝石+次氯酸盐”体系中的自由基状态。用生成的单氧630nm谱线记录了体系中自由基碎片的形貌。亚甲基深蓝染色法测定辐照前后细胞活力。结果表明，视频脉冲治疗的影响降低了肝细胞的活力百分比，激活了自由基过程，相应的增加了单氧生成。确定了这些过程与视频脉冲功率的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2000 10th International Crimean Microwave Conference. "Microwave and Telecommunication Technology". Conference Proceedings (IEEE Cat. No.00EX415)

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