Analysis of Parameters of Chromatographic Microcolumns Manufactured by Printing

Abstract

Based on the literary sources analysis, models of chromatographic microcolumns were developed. Models of these columns are printed using additive technology. We have carried out post-processing of these columns, their filling, performed an analysis of their sorption properties. It is shown that the characteristics of the obtained columns allow their use in microfluidic systems. We have chosen the following types of additive technologies for printing chromatographic columns: SLA (for creating a prototype) and SLM (for creating samples for research). We used software such as AutoCAD and Compass – 3D to design the column models. It is shown that the quality and accuracy of the manufactured columns depends on the choice of the software package. Printing a column prototype was carried out using the technology of polyurethane SLA-on by a 3D printer "EDEN 350". In the manufacture of the column was used technology PolyJet - layer-by-layer application of a photosensitive material and its subsequent curing with ultraviolet light. The use of additive technologies made it possible to optimize the geometry of the column, reduce its dimensions, create circular capillaries, manufacture additional elements for capillary fixation. Using SLM technology, powder steel column samples were printed. In this case was used the installation SLM 280HL, which implements the selective laser alloying of metal powder. The chromatographic properties of the prepared columns were investigated using a gas chromatograph "Crystal 5000.2" with a flame ionization detector at various pressures and temperature conditions. Investigations were conducted on natural gas (ethane, propane, isobutane, n-butane). The volume of the injected sample was 0.1 ml. Air was used as a carrier gas. Experiments have shown that when using a manufactured column, it is possible to separate the C2-C5 gas mixture in less than 2 minutes under the chromatograph mode: 125°C and 80 kPa.