A. Pogrebnjak, Ruslan Kimossov, Alexandr Myakinin, S. Plotnikov, A. Turlybekuly, S. Zhanyssov, A. Kistaubayeva, D. Shokataeva, A. Tsorayev
{"title":"Physical and Biological Properties of TiNbTa Alloy","authors":"A. Pogrebnjak, Ruslan Kimossov, Alexandr Myakinin, S. Plotnikov, A. Turlybekuly, S. Zhanyssov, A. Kistaubayeva, D. Shokataeva, A. Tsorayev","doi":"10.1109/NAP.2018.8915338","DOIUrl":null,"url":null,"abstract":"This work is devoted to obtaining a superelastic alloy with acceptable biomechanical parameters, investigation of mechanical properties and cytotoxicity. An ingot of TiNbTa alloy (titanium-niobium-tantalum) was smelted by two remelting in an arc furnace vacuum furnace. The alloy samples were analyzed by scanning electron microscopy (SEM) using the JSM-6390LV microscope with the energy dispersive microanalysis system INCA Energy Penta FET X3. The biocompatibility of composite materials under in vitro conditions was assessed by the following indices: viability level, adhesive properties, morphology, cell proliferation and differentiation. Cell viability was determined after 72 and 120 hours using the trypan blue dye exclusion test to selectively stain dead cells. The number of non-staining cells was counted using an electronic BID-RAD TC20 cell counter. Biological certification in vitro has shown that Ti-Nb-Ta alloys have no cytotoxic effect, have high biological activity and exhibit antibacterial effect, which allows them to be recommended for use in medicine.","PeriodicalId":239169,"journal":{"name":"2018 IEEE 8th International Conference Nanomaterials: Application & Properties (NAP)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2018 IEEE 8th International Conference Nanomaterials: Application & Properties (NAP)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/NAP.2018.8915338","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
This work is devoted to obtaining a superelastic alloy with acceptable biomechanical parameters, investigation of mechanical properties and cytotoxicity. An ingot of TiNbTa alloy (titanium-niobium-tantalum) was smelted by two remelting in an arc furnace vacuum furnace. The alloy samples were analyzed by scanning electron microscopy (SEM) using the JSM-6390LV microscope with the energy dispersive microanalysis system INCA Energy Penta FET X3. The biocompatibility of composite materials under in vitro conditions was assessed by the following indices: viability level, adhesive properties, morphology, cell proliferation and differentiation. Cell viability was determined after 72 and 120 hours using the trypan blue dye exclusion test to selectively stain dead cells. The number of non-staining cells was counted using an electronic BID-RAD TC20 cell counter. Biological certification in vitro has shown that Ti-Nb-Ta alloys have no cytotoxic effect, have high biological activity and exhibit antibacterial effect, which allows them to be recommended for use in medicine.
本工作致力于获得具有可接受的生物力学参数的超弹性合金,研究其力学性能和细胞毒性。采用电弧炉真空炉两次重熔法制备了一种TiNbTa合金(钛铌钽)锭。采用JSM-6390LV显微镜和INCA energy Penta FET X3能量色散微分析系统对合金样品进行扫描电镜(SEM)分析。通过活力水平、粘附性能、形态、细胞增殖和分化等指标评价复合材料的体外生物相容性。72h和120h后,用台盼蓝染色法对死亡细胞进行选择性染色,测定细胞活力。使用电子BID-RAD TC20细胞计数器计数未染色细胞的数量。体外生物鉴定表明,Ti-Nb-Ta合金无细胞毒作用,具有较高的生物活性和抗菌作用,可推荐用于医药领域。