Antimicrobial Potency of Methanolic Leaf Extracts from Selected Medicinal Plants against Staphylococcus aureus

Journal of Medical Microbiology and Diagnosis Pub Date : 2016-02-18 DOI:10.4172/2161-0703.1000219

Rachuonyo Ho, Ogola Pe, Arika Wm, Kiboi Ng, Wambani

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引用次数: 13

Abstract

The main aim of the study was to test for the antimicrobial potency of Aloe secundiflora, Bulbine frutescens, Tagetes minuta and Vernonia lasiopus against Staphylococcus aureus. All the plants showed a pronounced antimicrobial activity against Staphylococcus aureus with Tagetes minuta being the most active at low concentrations (MIC 8.9 mg/ml; MBC 10.0 mg/ml) whereas Vernonia lasiopus showing less activity (MIC 12.2 mg/ml; MBC 14.2 mg/ml). The efficacy test was carried out using the disc diffusion method. The standard antibiotics used were ciprofloxacin (5 μg/ml) and vancomycin (3 μg/ml) showed significant antimicrobial activity by producing zones of inhibition of 22 mm and 25 mm respectively. Dimethyl sulphoxide and distilled water were used as negative control. The extracts from the plants were also screened for the presence of phytochemicals with the results showing the presence of flavonoids, alkaloids, tannins and saponins in all the extracts. The study suggested that the selected medicinal plants can be used effectively in the treatment of bacterial infection caused by Staphylococcus aureus.

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部分药用植物甲醇叶提取物对金黄色葡萄球菌的抑菌作用

本研究的主要目的是研究芦荟、球根菜、万寿菊和水蛭对金黄色葡萄球菌的抑菌作用。所有植物对金黄色葡萄球菌均表现出明显的抑菌活性，其中万寿菊在低浓度(MIC 8.9 mg/ml)下抑菌活性最强;MBC 10.0 mg/ml)，而鼠眼鼠(Vernonia lasiopus)活性较低(MIC 12.2 mg/ml;MBC 14.2 mg/ml)。采用圆盘扩散法进行药效试验。标准抗生素为环丙沙星(5 μg/ml)和万古霉素(3 μg/ml)，抑菌带分别为22 mm和25 mm，抑菌活性显著。以二甲基亚砜和蒸馏水为阴性对照。对提取的植物进行化学成分筛选，结果表明，提取的植物中均含有黄酮类化合物、生物碱、单宁和皂苷。本研究提示所选药用植物可有效治疗金黄色葡萄球菌引起的细菌感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Medical Microbiology and Diagnosis

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