ISOLATION AND SCREENING OF INDIGENOUS MICROORGANISMS CAPABLE OF DEGRADING CELLULOSE TO TREAT HEMP HURD IN THANH HOA PROVINCE

SCIENTIFIC JOURNAL OF TAN TRAO UNIVERSITY Pub Date : 2023-02-11 DOI:10.51453/2354-1431/2022/876

K. Nguyen, Kieu Le, T. Du, T. Le

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Abstract

Hemp Hurd has not yet found an optimal treatment method, therefore, in this paper, we isolated and screened native microorganisms with high cellulose-degradation capability for potentially effective treatment. Native microorganisms were isolated on Gauze I and Hans, media then CMC-as activity was determined. Next, the species names of screening microorganisms were determined by total DNA isolation, PCR and Sequencing. The results showed that 02 bacterial strains and 06 actinomycete strains were selected from 32 isolates. Then, we optimized the growing conditions of the screened microorganisms. Among actinomycetes strains PU1.1, PU2.1 gave the highest weight loss results, therefore they were selected for further study. Our results showed that TG2.1 and CM4.1 are Bacillus subtilis. PU1.1 is Streptomyces lilacs, while PU1.2 is Streptomyces Misiones. The selected microorganisms belong to risk group level 1 (safety group). Thus, these strains can be used for further experiments in the research and production of microorganism-derived bio-products to treat hemp hurd in Thanh Hoa province.

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清化省大麻粪纤维素降解微生物的分离与筛选

Hemp Hurd尚未找到最佳的处理方法，因此，在本文中，我们分离并筛选了具有高纤维素降解能力的天然微生物，以进行潜在的有效处理。分别在纱布I和Hans培养基上分离原生微生物，测定CMC-as活性。然后，通过总DNA分离、PCR和测序确定筛选微生物的种名。结果表明，从32株分离菌株中筛选出02株细菌和06株放线菌。然后，对筛选出的微生物进行生长条件优化。在放线菌菌株PU1.1中，PU2.1的减重效果最好，因此选择该菌株进行进一步研究。结果表明，TG2.1和CM4.1为枯草芽孢杆菌。PU1.1为丁香链霉菌，PU1.2为Misiones链霉菌。所选微生物属于风险组1级(安全组)。因此，这些菌株可用于清化省微生物衍生生物制品的研究和生产。

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SCIENTIFIC JOURNAL OF TAN TRAO UNIVERSITY

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