UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEMANGI (Ocimumbasilicum L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcusaureus

Kesaktian Manurung, Jon Kenedy Marpaung, Mardianis Mardianis
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引用次数: 1

Abstract

Basil (Ocimumbasilicum L.) is one of the natural plants that is easily obtained in Asia such as Indonesia which has medicinal properties. Basil leaves contain flavonoid compounds, tannins, saponins, and steroids/triterpenoids. These compounds are known to have antibacterial activity. This study aims to determine the antibacterial activity of the ethanolic extract of basil leaves (Ocimumbasilicum L.) against Staphylococcus aureus bacteria. This research is experimental. The test was carried out through several stages including material collection, preparation of simplicia, making ethanol extract of basil leaves, and testing the inhibitory power of basil leaves against Staphylococcus aureus bacteria. The ethanol extract of basil leaves was made by the maceration method using 96% ethanol and concentrated with a rotary evaporator to obtain a thick extract from basil leaves. Antibacterial activity was tested by the agar diffusion method using disc paper. The results of the antibacterial activity test showed that the ethanolic extract of basil leaves inhibited the growth of Staphylococcus aureus bacteria at concentrations of extracts of 5%, 10%, 15%, 20%, and 25% with inhibition zone diameters of 8. 31 mm, 9. 43 mm, respectively. 9. 73 mm, 9. 91 mm, and 11. 33 mm. The ethanol extract of basil leaves has antibacterial activity against Staphylococcus aureus bacteria.
试验罗勒叶乙醇提取物(ocimusilicum L)的抗菌活性。对葡萄球菌的生长有影响
罗勒(Ocimumbasilicum L.)是一种在印度尼西亚等亚洲地区容易获得的天然植物,具有药用价值。罗勒叶含有类黄酮化合物、单宁、皂苷和类固醇/三萜。已知这些化合物具有抗菌活性。本研究旨在测定罗勒叶乙醇提取物对金黄色葡萄球菌的抑菌活性。这项研究是实验性的。本实验通过材料采集、单纯物制备、罗勒叶乙醇提取、罗勒叶对金黄色葡萄球菌的抑菌能力测试等几个阶段进行。采用96%乙醇浸渍法制备罗勒叶乙醇提取物,并用旋转蒸发器浓缩得到较浓的罗勒叶乙醇提取物。采用圆盘纸琼脂扩散法测定其抑菌活性。抑菌活性试验结果表明,罗勒叶乙醇提取物在5%、10%、15%、20%和25%浓度下均能抑制金黄色葡萄球菌的生长,抑菌带直径为8。31毫米,9毫米。分别为43毫米。9. 73毫米,9毫米。91毫米和11。33毫米。罗勒叶乙醇提取物对金黄色葡萄球菌具有抑菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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