Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone

2017 IEEE Healthcare Innovations and Point of Care Technologies (HI-POCT) Pub Date : 2017-11-01 DOI:10.1109/HIC.2017.8227629

F. Sun, Weili Chen, Hojeong Yu, Akid Omob, Ryan Brisbin, A. Ganguli, V. Vemuri, P. Strzeboński, Guangzhe Cui, K. J. Allen, Smit Desai, Weiran Lin, David M. Nash, D. Hirschberg, Ian Brooks, R. Bashir, B. Cunningham

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引用次数: 1

Abstract

New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single 15-μL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments.

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用微流控环介导的等温扩增和智能手机多路检测传染病

需要新的工具，以便在影响人类和动物健康的各种护理点应用中快速检测、识别和报告传染性病毒和微生物病原体。我们报告了一种用于疾病特异性DNA序列多路分析的平台的设计、构建和表征，该平台利用智能手机摄像头作为传感器，并结合手持仪器，将手机与硅基微流控芯片连接起来。以四种马呼吸道病原体的特异性核酸序列为例，我们展示了该系统在大约30分钟内使用单个15 μ l液滴检测样品进行选择性阳性/阴性序列测定的能力，包括综合实验对照。该系统达到了可与实验室方法和仪器相媲美的检测限。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2017 IEEE Healthcare Innovations and Point of Care Technologies (HI-POCT)

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