Microbiological Quality Assessment and Antibiogram of the Bacteria Isolated from Fish Feed, Oyo, South-West Nigeria

O. Olayiwola
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引用次数: 4

Abstract

Essential nutrient is required for the growth of aquaculture either in the wild or in the artificial environment. Therefore, artificial feed need to contain essential nutrients and free of pathogenic microorganism. Samples of both imported and local fish feed samples were collected and presence of microorganisms was examined using bacteriological media. Morphological and Biochemical identification of the isolates were carried out. Also, antibiotic susceptibility patterns of the bacteria isolated were carried out using Gram negative and positive specific antibiotics (Oxoid) with reference to zones of inhibition observed from the plates. It was observed that fish feed samples possess pH and moisture content ranges from 6.8 to 7.0 and 10% to 17% respectively. The proportion of microorganisms obtained from the sample shows Bacillus sp 33%, Staphylococcus sp 13%, Streptococcus sp 13%, Pseudomonas sp 7%, Klebsiella sp 7% and Proteus sp 6%. Antimicrobial susceptibility was tested and interpreted using NCCLS, 2006. Regulatory authorities on animal (fish) feed should therefore endeavor to ensure pathogen-free animal (fish) feed. This will ensure good and healthy meat or food (fish) production for human population.
尼日利亚西南部Oyo鱼饲料中分离细菌的微生物质量评价和抗生素谱分析
无论是在野外还是在人工环境中,水产养殖都需要必需的营养物质。因此,人工饲料需要含有必需的营养成分,不含病原微生物。本署收集了进口及本地鱼饲料样本,并利用细菌培养基检测是否有微生物存在。对分离菌株进行了形态和生化鉴定。此外,利用革兰氏阴性和阳性特异性抗生素(Oxoid)对分离的细菌进行抗生素敏感性分析,并参考从平板上观察到的抑制区。鱼饲料样品的pH值为6.8 ~ 7.0,水分含量为10% ~ 17%。从样品中获得的微生物比例为芽孢杆菌33%,葡萄球菌13%,链球菌13%,假单胞菌7%,克雷伯氏菌7%,变形杆菌6%。使用NCCLS, 2006进行抗菌药物敏感性测试和解释。因此,动物(鱼)饲料监管机构应努力确保动物(鱼)饲料不含病原体。这将确保为人类生产良好和健康的肉类或食品(鱼)。
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