REAL TIME POLYMERASE CHAIN REACTION (RT-PCR) FOR DNA PIG CONTAMINATION IDENTIFICATION SAUSAGE IN SIX DISTRICT PANDEGLANG BANTEN

International Journal Mathla’ul Anwar of Halal Issues Pub Date : 2021-03-15 DOI:10.30653/ijma.202111.13

Hadi Susilo

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Abstract

Sausage is a meat product processed that is popular food especially in Pandeglang, Banten Province. The importance of halal certificates or the existence of the MUI (Indonesian Ulama Council) halal logo for processed meat products makes Muslim people confident to consume them. The aim this research was to identify pig DNA contamination in sausage products in six districts in Pandeglang without the MUI halal labels using RT-PCR (Real Time-Polymerase Chain Reaction). RT PCR that can calculate to pig to fill these sample free from pig contamination. This research was divided into two stage, the first stage is extracted or carried out DNA and the second stage is RT PCR analysis. The results of the DNA purity test on sausage samples had DNA purity values of 1.84-1.9 (A260 / A280) and resulted in sample concentrations ranging from 37.8 to 102.5 ng / µl. The only amplification on the FAM curve was in the positive control pig. the Cq value ranges from 30 - 31.29. The results of RT PCR on sausage samples in the district area in Pandeglang Banten did not detect the presence of pig DNA.

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实时聚合酶链反应(rt-pcr)用于攀德郎六区猪污染鉴定香肠

香肠是一种肉制品加工，是受欢迎的食品，特别是在万丹省的攀德郎。清真证书的重要性或加工肉制品的MUI(印度尼西亚乌拉玛委员会)清真标志的存在使穆斯林有信心消费它们。本研究的目的是利用实时聚合酶链反应(RT-PCR)技术对没有MUI清真标签的板底郎6个区香肠产品中的猪DNA污染进行鉴定。RT - PCR可以计算到猪填充这些样品没有猪的污染。本研究分为两个阶段，第一阶段提取或进行DNA，第二阶段进行RT - PCR分析。香肠样品的DNA纯度检测结果为1.84 ~ 1.9 (A260 / A280)，样品浓度为37.8 ~ 102.5 ng /µl。FAM曲线上唯一扩增的是阳性对照猪。Cq的取值范围是30 ~ 31.29。对万丹市攀德郎地区香肠样品进行RT - PCR检测，未检测到猪DNA的存在。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal Mathla’ul Anwar of Halal Issues

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