Cell analysis system using a filter-free fluorescence sensor

Abstract

We proposed a cell analysis system without optical filters using a filter-free fluorescence sensor which used wavelength dependent absorption depth in a silicon substrate. Excitation light and fluorescence from fluorescently labeled cells were separated and their light intensities were measured at the same time by applying the proposed parameter. This result shows the filter-free fluorescence sensor can be used to detect cell labeled with various fluorescence reagents. The measured fluorescence of cells was good agreement with fluorescence intensity observed by conventional fluorescence microscopy.