Administration of olive oil optimizes acetic acid induced colitis in CD1 mice

Abstract

INTRODUCTION Inflammatory bowel diseases (IBD), including Crohn’s disease and ulcerative colitis (UC) have a high incidence in industrialized nations and severely affect the life quality of patients (Amosy and Koma 2013). These diseases are defined clinically through inflammatory disorder in gastrointestinal tract accompanied with diarrhea, abdominal pains, weight loss, nausea and pathological changes such as a loss of mucosal integrity and inflammatory cell infiltration (Xavier et al., 2007). In addition, the presence of white blood cells. at the intestinal mucosal tissue is characteristic feature of this disease (Wang et al., 2005). Colitis affects the distal colon portion and induces non-transmural inflammation, massive necrosis of mucosal and Colitis is a chronic disease that affects individuals at different ages and affects the quality of their life. So far, there are three different experimental models for colitis. However, animals in these models die quickly duo to the associates toxicity. The aim of this study is to optimize acetic acid induced acute colitis mouse model with olive oil co-administration. Male albino CD1 mice (n= 91) were randomly divided into 13 groups (n= 7/group). To induce colitis, mice were administrated with different volumes (100 or 200 μl) and concentrations (1%, 2% and 4%) of acetic acid by intrarectal (IR) injection either for three consecutive days or every other day for a total of 6 days. Olive oil (1 μL) was co-injected with acetic acid. One day later, mice were sacrificed and colon tissues were fixed in 10% formalin for histological examinations. The induction of colitis by acetic acid was dependent on both the volume and the concentration of acetic acid. Interestingly, co-administration of olive oil with different volumes and concentrations of acetic acid, although did not alter the severity of inflammation, it increased the animal survival rate and decreased the associated toxicity. Injection of co-administration of olive oil with acetic acid is a new modification to enhance the application of this model. Article history: