Uji Sitotoksik Ekstrak Etanol Herba Rumput Akar Wangi (Polygala paniculata L.) Terhadap Sel Kanker Widr Secara In Vitro

Abstract

The development of cancer treatment using natural ingredients has recently increased. This is because many of the active substances are found in nature, especially in plants, have many substances that can interrupt abnormal growth cells. The Polygala paniculata herbs have become a concern to be developed into potential anticancer candidates originating from nature. This study aims to take measurements of the Polygala paniculata L. ethanolic extract cytotoxic activity on WiDr cancer cells based on IC50 values. Absolute ethanol was used as a solvent to extracting it by maceration method. The Polygala paniculata L. herbs ethanol extract were carried for the cytotoxic assay by the MTT method (3(4,5-dimethylthiazol-2il) -2,5-diphenyl tetrazolium bromide) on WiDr cells. The extract was tested with a concentration of 31.25 μg / ml; 62.5 μg / ml; and 125 μg / ml; 250 μg / ml; 500 μg / ml. The result of cytotoxic activity assay at IC50 80,52 μg / ml was less than 100 ppm. The results showed that Polygala paniculata herb ethanolic extract had a potential as an alternative of chemotherapy.