Hyperspectral Colon Tissue Classification using Morphological Analysis

Abstract

Diagnosis and cure of colon cancer can be improved by efficiently classifying the colon tissue cells into normal and malignant classes. This paper presents the classification of hyperspectral colon tissue cells using morphological analysis of gland nuclei cells. The application of hyperspectral imaging technique in medical image analysis is a new domain for researchers. The main advantage in using hyperspectral imaging is the increased spectral resolution and detailed subpixel information. Biopsy slides with several microdots, where each microdot is from a distinct patient, are illuminated with a tuned light source and magnification is performed up to 400times. The proposed classification algorithm combines the hyperspectral imaging technique with linear discriminant analysis. Dimensionality reduction and cellular segmentation is achieved by independent component analysis (ICA) and k-means clustering. Morphological features, which describe the shape, orientation and other geometrical attributes, are next to be extracted. For classification, LDA is employed to discriminate tissue cells into normal and malignant classes. Implementation of LDA is simpler than other approaches; it saves the computational cost, while maintaining the performance. The algorithm is tested on a number of samples and its applicability is demonstrated with the help of measures such as classification accuracy rate and the area under the convex hull of ROC curves