Single-Cell Transcriptomics Reveals Tissue Architecture in Ovarian Carcinosarcoma

Junfen Xu, Y. Cen, Weiguo Lu
{"title":"Single-Cell Transcriptomics Reveals Tissue Architecture in Ovarian Carcinosarcoma","authors":"Junfen Xu, Y. Cen, Weiguo Lu","doi":"10.21203/rs.3.rs-986007/v1","DOIUrl":null,"url":null,"abstract":"\n Background\n\nOvarian carcinosarcoma (OCS) is one of rarest and most challenging histologic subtype of ovarian cancer. It features remarkable cellular heterogeneity. Using single-cell RNA sequencing, we characterize the cellular composition of the OCS and identify their molecular characteristics.\nMethods\n\nwe applied single-cell RNA sequencing (scRNA-seq) to resected primary OCS for the in-depth analysis of tumor cells and the TME. Immunohistochemistry (IHC) staining was used for validation.\nResults\n\nMalignant epithelial and fibroblast cells displayed a high-degree of intratumoral heterogeneity. We revealed that certain epithelial cell subclusters had high levels of drug resistance scores and many active metabolic pathways. Furthermore, γδ T cells exhibited enriched IFNγ and IFNα response characteristics. Notably, we observed that macrophages were mainly M2-like macrophages with immunosuppressive properties. In addition, we found that the CD1A+/FCER1A+ DC cells were enriched with genes related to cytolytic effector pathway. Analyzing ligand-receptor interaction pairs between cell types, we identified broadly interacting cells and observed an interaction between the ANXA1+ epithelial population and FPR1+/FPR3+ myeloid cells.\nConclusion\n\nOur findings provide a comprehensive single-cell transcriptomic landscape of human OCS and present a well-established resource for elucidating OCS diversity.","PeriodicalId":192166,"journal":{"name":"Proceedings of the ASGO 2022 7th International Workshop","volume":"13 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the ASGO 2022 7th International Workshop","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21203/rs.3.rs-986007/v1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background Ovarian carcinosarcoma (OCS) is one of rarest and most challenging histologic subtype of ovarian cancer. It features remarkable cellular heterogeneity. Using single-cell RNA sequencing, we characterize the cellular composition of the OCS and identify their molecular characteristics. Methods we applied single-cell RNA sequencing (scRNA-seq) to resected primary OCS for the in-depth analysis of tumor cells and the TME. Immunohistochemistry (IHC) staining was used for validation. Results Malignant epithelial and fibroblast cells displayed a high-degree of intratumoral heterogeneity. We revealed that certain epithelial cell subclusters had high levels of drug resistance scores and many active metabolic pathways. Furthermore, γδ T cells exhibited enriched IFNγ and IFNα response characteristics. Notably, we observed that macrophages were mainly M2-like macrophages with immunosuppressive properties. In addition, we found that the CD1A+/FCER1A+ DC cells were enriched with genes related to cytolytic effector pathway. Analyzing ligand-receptor interaction pairs between cell types, we identified broadly interacting cells and observed an interaction between the ANXA1+ epithelial population and FPR1+/FPR3+ myeloid cells. Conclusion Our findings provide a comprehensive single-cell transcriptomic landscape of human OCS and present a well-established resource for elucidating OCS diversity.
单细胞转录组学揭示卵巢癌肉瘤的组织结构
卵巢varian癌肉瘤(OCS)是卵巢癌中最罕见和最具挑战性的组织学亚型之一。它具有显著的细胞异质性。利用单细胞RNA测序,我们表征了OCS的细胞组成,并鉴定了它们的分子特征。方法采用单细胞RNA测序(scRNA-seq)技术对切除的原发OCS进行肿瘤细胞及TME的深入分析。采用免疫组织化学(IHC)染色进行验证。结果恶性上皮细胞和成纤维细胞表现出高度的瘤内异质性。我们发现某些上皮细胞亚群具有高水平的耐药评分和许多活跃的代谢途径。此外,γδ T细胞表现出丰富的IFNγ和IFNα反应特征。值得注意的是,我们观察到巨噬细胞主要是具有免疫抑制特性的m2样巨噬细胞。此外,我们发现CD1A+/FCER1A+ DC细胞富含与细胞溶解效应通路相关的基因。通过分析细胞类型之间的配体-受体相互作用对,我们确定了广泛相互作用的细胞,并观察到ANXA1+上皮细胞群与FPR1+/FPR3+骨髓细胞之间的相互作用。结论本研究提供了一个完整的人类OCS单细胞转录组图谱,为阐明OCS多样性提供了一个完善的资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信