Evaluating cytotoxic effect of the extracted compounds from Ehretia asperula Zoll. & Mor stem on several cancer cell lines

TAP CHI SINH HOC Pub Date : 2018-11-06 DOI:10.15625/0866-7160/V40N2.12955

Vu Thi Nguyet, N. Dat, L. Huong, Tran Thị Hong Ha, Nguyen Hong Chuyen, N. Hang, Dang Dinh Kim

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引用次数: 1

Abstract

This paper reports the cytotoxic effect on several cancer cell lines of the stem extracts and of some isolated compounds from Ehretia asperula. All the extracts exhibited cytotoxic effects on at least one cancer cell line. The n-hexane extract showed potent cytotoxic activity on Hep-G2, MCF-7 and HeLa cell lines with IC50 values of 28.3 g/ml, 14.42 g/ml and 18.59 g/ml, respectively, while the methanolic, ethyl acetate and water extracts exhibited toxicity towards MCF-7 cells with IC50 values of 16.45 g/ml, 13.4 g/ml and 39.78 g/ml, respectively. 06 compounds have been isolated from the ethyl acetate fraction of Ehretia asperula stem. Methyl caffeate has a strong cytotoxicity against Hep-G2, HeLa and MCF-7 cancer cell lines with IC50 values of 2.83 g/ml, 3.38 g/ml and 4.4 g/ml, respectively. Oresbiusin B was active against Hep-G2 with IC50 value of 9.89 g/ml. The other compounds including coniferaldehyde, 9′-methoxydehydrodiconiferyl alcohol and vanillic acid did not have any cytotoxic effect on the tested cancer cell lines. So, the obtained results have suggested possibility of using the potential Ehretia asperula extracts as health food for preventing and curing cancer diseases. Keywords: Ehretia asperula, methyl caffeate, oresbiusin B, Cancer cell lines. Citation: Vu Thi Nguyet, Nguyen Tien Đat, Le Mai Huong, Tran Thi Hong Ha, Nguyen Hong Chuyen, Nguyen Thi Hang4, Đang Đinh Kim, 2018. Evaluating cytotoxic effect of the extracted compounds from ehretia asperula zoll. & mor stem on several cancer cell lines.Tap chi Sinh hoc, 40(2): 145153. https://doi.org/10.15625/0866-7160/v40n2.12955. *Corresponding author: dangkim.iet@gmail.com

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曲霉Ehretia asperula Zoll提取化合物的细胞毒作用评价。&更多的干细胞在几种癌细胞系上

本文报道了黑线Ehretia asperula茎提取物和一些分离化合物对几种癌细胞的细胞毒作用。所有提取物都对至少一种癌细胞系表现出细胞毒性作用。正己烷提取物对Hep-G2、MCF-7和HeLa细胞的IC50值分别为28.3g/ml、14.42g/ml和18.59g/ml，甲醇、乙酸乙酯和水提取物对MCF-7细胞的IC50值分别为16.45g/ml、13.4g/ml和39.78g/ml。从黑线Ehretia asperula茎的乙酸乙酯部位分离得到了06个化合物。咖啡酸甲酯对Hep-G2、HeLa和MCF-7癌细胞具有较强的细胞毒性，IC50值分别为2.83g/ml、3.38g/ml和4.4g/ml。Oresbiusin B对Hep-G2有抑制作用，IC50值为9.89g/ml。其他化合物包括松柏醛、9′-甲氧基脱氢二针叶醇和香草酸对所测试的癌细胞系没有任何细胞毒性作用。因此，本研究结果为利用有潜力的黑道Ehretia asperula提取物作为防治癌症疾病的保健食品提供了可能性。关键词:曲霉Ehretia asperula，咖啡酸甲酯，resbiusin B，癌细胞系引用本文:Vu Thi Nguyet, Nguyen Tien Đat, Le Mai Huong, Tran Thi Hong Ha, Nguyen Hong Chuyen, Nguyen Thi Hang4， Đang Đinh Kim, 2018。评价黄曲霉ehretia asperula zoll提取化合物的细胞毒作用。&更多的干细胞在几种癌细胞系上。科学通报，40(2):145 - 153。https://doi.org/10.15625/0866-7160/v40n2.12955。*通讯作者:dangkim.iet@gmail.com

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