Changes in the cellular composition of guinea pig’s distal airways epithelium in the dynamics of experimental ovalbumin-induced allergic inflammation

Reports of Morphology Pub Date : 2021-09-23 DOI:10.31393/morphology-journal-2021-27(3)-08

S. Popko

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引用次数: 1

Abstract

The problem of studying the processes of restructuring of airways epithelium of humans and animals of adaptive nature under the influence of various factors on the body remains the subject of scientific discussions. The aim of this work is to study morphometric changes in the cellular composition guinea pig’s distal airways epithelium in the dynamics of experimental ovalbumin-induced allergic inflammation. We studied lung of 48 male guinea pigs, using histological, morphometric and statistical methods, under conditions of experimental ovalbumin-induced allergic inflammation, simulated by subcutaneous sensitization and subsequent intranasal inhalation with ovalbumin. The average number of epithelial cells of small bronchi and terminal bronchioles was determined: basal epithelium cells, ciliated cells, goblet cells and exocrine bronchiolar cells per unit area of 10000 μm2. We have shown the most significant reactive morphometric changes on the 23rd and 30th days of the experiment. We demonstrated a decrease of the number of basal cells (by 1.5 times compared to the control, p*/**<0.01) and ciliated cells (by 1.6 times compared to the control, p*/**<0.001) and an elevation of the average number of goblet cells (by 2.6 times compared to the control, p*/**<0.01) in bronchioles, a decrease of the average number of exocrine bronchiolar cells (by 1.6 times compared to the control, p*/**<0.01) in terminal bronchioles. These changes are the morphological confirmation of the development of bronchial hyperreactivity as a result of the action of the allergen. Sensitization and allergization with ovalbumin cause statistically significant morphological changes in the cellular composition of small bronchi and terminal bronchioles of an alterative nature in the early period and adaptive – in the late period of allergic inflammation, corresponding to the main morphological manifestations of allergic inflammation.

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豚鼠远端气道上皮细胞组成在卵清蛋白诱发的实验性过敏性炎症中的动态变化

研究人和动物气道上皮细胞在各种机体因素影响下的适应性重组过程仍然是科学界讨论的主题。这项工作的目的是研究豚鼠远端气道上皮细胞组成在卵清蛋白诱导的实验性过敏性炎症动态变化中的形态计量学变化。我们使用组织学、形态计量学和统计学方法研究了 48 只雄性豚鼠在卵清蛋白诱导的实验性过敏性炎症条件下的肺部情况，这种过敏性炎症是通过皮下致敏和随后鼻内吸入卵清蛋白来模拟的。测定了小支气管和末端支气管上皮细胞的平均数量：每 10000 μm2 单位面积的基底上皮细胞、纤毛细胞、鹅口疮细胞和支气管外分泌细胞。我们在实验的第 23 天和第 30 天发现了最明显的反应性形态变化。我们发现基底细胞（与对照组相比减少了 1.5 倍，p*/**<0.01）和纤毛细胞（与对照组相比减少了 1.6 倍，p*/**<0.001）的数量减少了，而小管细胞的平均数量增加了（与对照组相比增加了 2.6 倍，p*/**<0.001）。6倍，p*/**<0.01），终末支气管的外分泌支气管细胞平均数量减少（比对照组减少1.6倍，p*/**<0.01）。这些变化从形态上证实了过敏原作用下支气管高反应性的发展。卵清蛋白致敏和过敏会导致小支气管和末端支气管细胞组成发生统计学意义上的显著形态学变化，在过敏性炎症的早期为改变性变化，在晚期为适应性变化，这与过敏性炎症的主要形态学表现是一致的。

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Reports of Morphology

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