Determination of IgG and IgM against COVID- 19 for Recovered Patients at Different Intervals and by Different Techniques

Enass Al Hadidi, Mahmood A. Al-Tobje
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Abstract

The research aimed to determine the concentrations of IgG and IgM antibodies for different intervals after recovery from Covid-19, namely 4, 6 and 8 months; the research also aimed to identify the accordance between two types of immunity techniques used to identify the existence of antibodies. The results showed the existence of IgG antibodies with a percentage of (94.03%), and IgM antibodies with a percentage of 55.22% in individuals under study when using ELISA technique. The percentages of these antibodies were (86.56%) for IgG and (16.42%) for IgM when using Rapid test cassette in diagnosis; the matching was 67% between the two methods. Samples of the control group also showed the presence of IgG and IgM with percentages of 68% and 88% respectively.The average concentrations of IgG antibodies were 33.05, 43.21 and 37.53 after 4, 6 and 8 months after infection respectively; the peak was at the 6th month after infection. The averages of IgM were 14.45, 18.52 and 19.18 after 4, 6 and 8 months of being infected respectively; the peak was at the 8th month after infection.
不同时间间隔、不同技术测定康复患者抗COVID- 19 IgG和IgM水平
本研究旨在检测新冠肺炎恢复后4、6、8个月不同时间段的IgG和IgM抗体浓度;该研究还旨在确定用于识别抗体存在的两种免疫技术之间的一致性。ELISA检测结果显示,IgG抗体的存在率为94.03%,IgM抗体的存在率为55.22%。使用快速检测盒诊断时,IgG抗体阳性率为86.56%,IgM抗体阳性率为16.42%;两种方法的匹配度为67%。对照组样品中IgG和IgM的存在率分别为68%和88%。感染后4个月、6个月和8个月IgG抗体平均浓度分别为33.05、43.21和37.53;感染后6个月为高峰。感染后4个月、6个月和8个月IgM平均值分别为14.45、18.52和19.18;发病高峰在感染后第8个月。
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